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EC number: 943-175-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-05-05 to 2015-08-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13th April, 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The respective volumes of the aqueous samples (Level 1: 1.5 mL; level 2: 2 mL; level 3 0 4 mL; level 4: 0.2 mL; level 5 0.2 mL were diluted with copper free water ad 10 mL (level 1 3 and 4), 20 mL (level 5) or 25 mL (level 2), respectively.
- Sampling method: 0.5 mL of each dilution was mixed with 0.5 mL analytical grade methanol and measured by LC-MS/MS.
- Sample storage conditions before analysis: Measured directly. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The highest nominal concentration was prepared by adding the required nominal amount of the test item (200 mg/L test item) and stirring for at least 15 minutes at room temperature. To obtain the other required test concentration the highest assay was serially diluted with dilution water and thoroughly mixed, sampled for later chemical analysis and distributed to the test beakers. The test solutions were freshly prepared before test start.
- Controls: Promary controlwith K2Cr2O7 as reference and one further control consisted of dilution water only.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: clone V
- Source: German Federal Environment Agency, lnstitut fi.'ir Wasser-, Boden und Lufthygiene. Specimens used in the test were bred in the laboratory of the Fraunhofer IME.
- Age of parental stock (mean and range, SD): 3 weeks old
- Feeding during test: no
ACCLIMATION
- Acclimation period: 1 week
- Acclimation conditions (same as test or not): no, room temperature in ca. 1.8 L dilution water
- Type and amount of food: algal suspension of Desmodesmus subspicatus
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality observed
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- In spite of the passage through a lime-stone column, hardness was below the recommended (not required) range (140 - 250 mg/L as CaCO3) for Daphnia magna indicated in the guideline. Historical data showed values of 70 — 120 mg/L as CaCO3. Total hardness: 1.1 mmol/L.
- Test temperature:
- 18-22°C
- pH:
- 7.6 - 8.3
- Dissolved oxygen:
- 8.1 - 8.7 mg/L
- Conductivity:
- 231 µS/cm
- Nominal and measured concentrations:
- Nominal concentrations: 12.5, 25, 50, 100, 200 mg/L, recovery of the test substance was analytically determined and was out of the 80-120 % range, therefore the geometric means were calculated: 7.74, 19.6, 45.0, 94.6, and 196 mg/L a.s.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Type: closed
- Material, size, headspace, fill volume: glass, 50 mL nominal volume, filled with about 50 mL test solution.
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified drinking water was used as holding- and dilution water. The purification included filtration with activated charcoal, passage through a limestone column, and aeration.
- Total organic carbon: 0.41 mg/L
- Alkalinity: 1.6 mmol/L
- Ca/mg ratio: 1.0/0.1
- Conductivity: 231 µS/cm
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8
- Light intensity: 779-807 lx (corresponding to 10.3 — 10.8 µE/(m²s)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The numbers of dead animals were visually determined daily and dead specimens (if occurred) were removed. Any abnormalities (if occurred) in appearance and behaviour were recorded. immobility was determined according to the OECD guideline 202. Specimens which were not able to swim within 15 seconds after gentle agitation of the test vessel were considered to be immobilised (even if they were still moving their antennae).
- Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 196 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 94.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: no
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 24 h EC50 Value Immobilisation: 0.93 mg/L (95% CL: 0.83-1.04) - Reported statistics and error estimates:
- For immobilisation, the NOEC and LOEC were determined. EC10, EC20, and EC50 values together with 95 % confidence intervals (where possible) were calculated by Probit-analysis assuming log-normal distribution of the values. All statistical calculations were performed using the computer programme ToxRat®.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the present study the NOEC and EC50 values in Daphnia magna for Rhamnolipids according to OECD Test giuideline 202 were determined. Since no mortality and no immobilisation occurred during the first 24 h of the test and immobilisation was only detected at the highest concentration applied after 48 h the substance was considered to be not acutely toxic to Daphnia magna until 94.6 mg/L (NOEC).
- Executive summary:
The 48 hr-acute toxicity of Rhamnolipids to Daphnia magna was studied under static conditions according to PECD Guideline 202 (2004) and EU method C.2 (2008). Daphnids were exposed to control, and test chemical at nominal concentrations of 12.5, 25, 50, 100, 200 mg/L (10.5, 21.0, 42.0, 84.0, and 168 mg a.i./L). Mortality/immobilization and sublethal effects were observed daily.
The mean measured test item concentrations of the freshly prepared test solution of the active substance (initial concentrations) of the test item were between 84.2 % and 116 % of nominal concentrations, and in the aged test solution between 64.6 % and 117 %. Due to recovery rates were not in a range of 80 - 120 % of nominal throughout the test, geometric means were applied for effect assessment. The geometric measured concentrations were 7.74, 19.6, 45.0, 94.6, and 196 mg a.s./.
No significantly increased mortality (NOEC) up to a concentration of 94.6 mg a.i./L was detected when compared to the control daphnids. The EC50 was estimated to be > 196 mg a.i./L.
Results Synopsis
Test Organism Age: Daphnia magna <24 h
Test Type: Static
EC50: > 196mg a.i./L
Endpoint(s) Effected: mortality
Reference
NOEC and EC50 values for immobilisation at test end [mg a.s./L].
NOEC = No observed effect concentration; LOEC = Lowest obsen/ed effect concentration;
EC = Effect concentration; CL = Confidence level; n.d. = not determined due to mathematical
reasons; effect concentrations given as Qometric mean values.
|
Mortality |
NOEC |
94.6 |
LOEC |
196 |
EC10 (95% CL) |
111 (n.d.) |
EC20 (95% CL) |
140 (n.d.) |
EC50 (95% CL) |
> 196 |
Validity criteria:
- With 0% the percent mortality observed in the controls over the test period is </= 10%.
- The dissolved oxygen concentration at the end of the aging period (48 h) was >/= 3 mg/L in control and test vessels.
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 196 mg/L
Additional information
The short-term screening-level data for aquatic toxicity determined EC50 (daphnids) > 196 mg/L.
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