Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 280-489-7 | CAS number: 83567-04-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From July 12, 1982 to July 26, 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- in unwashed eyes the duration of te observation period is insufficient; in washed eyes, the study is acceptable
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Principles of method if other than guideline:
- Nine New-Zealand white rabbits received 100 mg of the test substance in the conjunctival sac of one eye. The other eye served as negative control. The treated eyes of 6 animals remained unwashed and eyes of the other 3 animals were washed after application. Signs of ocular irritation were examined at 1, 24, 48, 72 and 96 h and at 1 and 2 weeks after treatment. The grading and scoring of irritating reactions was performed in accordance with the Draize scale.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Trisodium 7-[[4-chloro-6-[ethyl[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(4-methoxy-2-sulphonatophenyl)azo]naphthalene-2-sulphonate
- EC Number:
- 280-489-7
- EC Name:
- Trisodium 7-[[4-chloro-6-[ethyl[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(4-methoxy-2-sulphonatophenyl)azo]naphthalene-2-sulphonate
- Cas Number:
- 83567-04-8
- Molecular formula:
- C30H28ClN7O14S4.3Na C30H25ClN7Na3O14S4
- IUPAC Name:
- trisodium 7-[[4-chloro-6-[ethyl[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(4-methoxy-2-sulphonatophenyl)azo]naphthalene-2-sulphonate
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Origin: Nihon Dobutsu Co., Osaka
Body weight: 2.28 - 3.17 kg
Temperature and relative humidity: 24+/-2°C and 60+/-10%, respectively
Lighting time: 12 h daily
Food: CG-3 type (100 g/day)
Water: tap water, ad libitum
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 100 mg on the everted lower lid
- Duration of treatment / exposure:
- Unwashed group: NA
Washed group: 30 seconds - Observation period (in vivo):
- 14 d
- Number of animals or in vitro replicates:
- Unwashed group: 6
Washed group: 3 - Details on study design:
- Nine New-Zealand white rabbits received 100 mg of the test substance in the conjunctival sac of one eye. The other eye remained untreated and served as negative control. The treated eyes of 6 animals remained unwashed and eyes of the other 3 animals were washed after application. Reading of ocular lesions was conducted at 1, 24, 48, 72 and 96 h and at 1 and 2 weeks after treatment. The grading and scoring of irritating reactions was performed in accordance with the Draize scale.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): in 3 animals for 1 minute with 300 mL lukewarm water
- Time after start of exposure: 30 seconds
SCORING SYSTEM: Draize
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Remarks:
- washed
- Basis:
- mean
- Remarks:
- 3 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- cornea opacity score
- Remarks:
- unwashed
- Basis:
- mean
- Remarks:
- 6 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0.83
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 14 d
- Irritation parameter:
- iris score
- Remarks:
- washed
- Basis:
- mean
- Remarks:
- 3 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Remarks:
- unwashed
- Basis:
- mean
- Remarks:
- 6 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Remarks:
- washed
- Basis:
- mean
- Remarks:
- 3 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Remarks:
- unwashed
- Basis:
- mean
- Remarks:
- 6 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 h
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Remarks:
- washed
- Basis:
- mean
- Remarks:
- 3 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Remarks:
- unwashed
- Basis:
- mean
- Remarks:
- 6 rabbits
- Time point:
- 24/48/72 h
- Score:
- 0.17
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- The test substance produced some grade of irritant reactions such as cornea opacity and hyperemia, chemosis and discharge in conjunctiva in unwashed eyes at 1 or 24 h after application. In the washed eyes, no irritant reaction was observed in the animals. The mean total score of these irritant reactions for the unwashed group was maximum at 24 h after application and the score was 11.2. Although slight cornea opacity remained in 4/6 unwashed animals after 14 d, other irritant reactions disappeared within 2 to 7 d after application in all remaining animals.
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- Under the study conditions, the substance was considered not to be irritating to eyes if the substances is washed out of the eyes. If the substance is not washed out, slight cornea opacity remained for 14 days in 4 of 6 rabbits showing a tendency for reversibility in the areas affected.
- Executive summary:
A study was conducted to determine the eye irritation potential of the substance according to an internal method of the laboratory. Nine New-Zealand white rabbits received 100 mg of the test substance in the conjunctival sac of one eye. The other eye remained untreated and served as negative control. The treated eyes of 6 animals remained unwashed and eyes of the other 3 animals were washed after application. Signs of ocular irritation were examined at 1, 24, 48, 72 and 96 h and at 1 and 2 weeks after treatment. The grading and scoring of irritating reactions was performed in accordance with the Draize scale. The test substance produced irritant reactions such as corneal opacity and hyperemia, chemosis and discharge in the conjunctiva of unwashed eyes at 1 and 24 h after application. In the washed eyes, no irritant reaction was observed in the animals. The mean total score of these irritant reactions for the unwashed group was 11.2 at 24 h. Slight corneal opacity remained in 4 out of 6 unwashed animals at 14 d, other irritant reactions disappeared within 2 to 7 d after application in all remaining animals. (Hosokawa, 1982).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.