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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian cell study: DNA damage and/or repair
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
The induction of bacterial mutation and hepatocyte unscheduled DNA synthesis by monosubstituted anilines
Author:
Thompson, C.Z.; Hill, L.E.; Epp, J.K.; Probst, G.S.
Year:
1983
Bibliographic source:
Environmental Mutagenesis 5(6), pp. 803-811

Materials and methods

Principles of method if other than guideline:
- Principle of test: Hepatocyte Unscheduled DNA Synthesis (UDS) as described in:

Probst GS, McMahon RE, Hill LE, Thompson CZ, Epp JK, Neal SB (1981): Chemically-induced unscheduled DNA synthesis in primary rat hepatocyte cultures: A comparison with bacterial mutagenicity using 218 compounds. Environ Mutagen 3: 11-32
GLP compliance:
not specified
Type of assay:
unscheduled DNA synthesis

Test material

Constituent 1
Chemical structure
Reference substance name:
2'-aminoacetophenone
EC Number:
209-002-8
EC Name:
2'-aminoacetophenone
Cas Number:
551-93-9
Molecular formula:
C8H9NO
IUPAC Name:
1-(2-aminophenyl)ethan-1-one
Test material form:
solid: crystalline

Test animals

Species:
rat
Strain:
Fischer 344
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 150-170 g

Administration / exposure

Route of administration:
not specified
Vehicle:
- Vehicle(s)/solvent(s) used: DMSO
Duration of treatment / exposure:
not specified
Frequency of treatment:
not specified
Post exposure period:
not specified
Doses / concentrationsopen allclose all
Dose / conc.:
0.5 other: nmol/mL
Remarks:
lower limit of test concentration range
Dose / conc.:
1 000 other: nmol/mL
Remarks:
upper limit of test concentration range
No. of animals per sex per dose:
not specified
Control animals:
yes
Positive control(s):
2-acetylaminofluorene; N-methyl-N-nitro-N-nitrosoguanidine

Examinations

Tissues and cell types examined:
hepatocytes
Evaluation criteria:
not specified
Statistics:
not specified

Results and discussion

Test results
Key result
Sex:
male
Genotoxicity:
negative
Toxicity:
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not examined
Positive controls validity:
valid

Any other information on results incl. tables

Hepatocyte UDS
Active range [nmol/mL] Concentration counted (*) [nmol/mL] Nuclear grain counts(**)
Treated Untreated Interpretation
None 1000 -0.9 ± 1.0 -1.2 ± 1.2 negative

* Results for the highest nontoxic concentration or the most active concentration for inducing UDS are reported.

** Nuclear silver grain counts obtained from 20 morphologically unaltered cells after incubation of hepatocytes with test compound for 20 hr (mean  ± SD).

Applicant's summary and conclusion

Conclusions:
The substance was reported to be negative (not genotoxic) in an unscheduled DNA synthesis (UDS) in rat hepatocyte culture at up to 1000 µg/mL.