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EC number: 701-122-3 | CAS number: 106185-75-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- all validity criteria of the test have been fulfilled . Minor deviation was considered not to affect the results of the test as no impact was observed on the inoculum blank respiration throughout the duration of the test
- Deviations:
- yes
- Remarks:
- Temperatures recorded continuously were situated between 20.7 and 22.4°C which was slightly higher than the requirements as laid down in the study plan (22 °C ± 2 °C and constant within 1 °C)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 5 March 2015
- Specific details on test material used for the study:
- Physico-chemical Properties:
Specific Gravity: 0.916 at 20°C
Vapour Pressure: 0.0424 Pa (25 °C)
Solubility in water: 25.83 mg.L-1 (Slow-stirring method)
log Kow: 4.4 at 35°C (HPLC Method) - Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: river water
- Details on inoculum:
- Origin: River water was sampled from “La Mourachonne”, situated downstream of a plant treating domestic wastewater (Pegomas, France).
Pre-conditioning: The river water was aerated for about 7 days before use as inoculum at the test temperature to reduce endogenous respiration. - Duration of test (contact time):
- ca. 56 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Test water: Mineral medium, Reconstituted water, as prescribed by the OECD Guideline No. 301
Composition of the mineral medium : KH2PO4 (8.50 g/L), K2HPO4 ( 21.75 g/L), NA2HPO4. 2H2O (33.40 g/L), NH4CL (0.50 g/L) CaCl2. 2H2O (36.40 g/L), MgSO4. 7H2O (22.50 g/L), FeCl3. 6H2O (0.25 g/L).
Test environment: Controlled environment cabinet (22°C ± 2°C); the test was performed in the dark.
TEST SYSTEM
Culture apparatus: All-glass sealed with ground glass stoppers BOD (Biological Oxygen Demand) bottles of approximately 300 mL capacity.
Inoculum: A concentration of inoculum of approximately 10^5 cells.L-1 was used.
Number of culture flasks/concentration: 18 bottles containing test item, inoculum, mineral medium and silica gel (test suspension)
SAMPLING
Determinations of the concentration of dissolved oxygen using an oxygen electrode in duplicate vessels were made at day 0, 7, 14, 21, 29, 35, 42, 49 and 56 for the “test suspension” and “inoculum blank with silica gel” series, at day 0, 7, 14, 21 and 29 for the “procedure control” and “inoculum blank without silica gel” series, and only at the start of the test and after 7 and 14 days of incubation for the “toxicity control” series.
Temperature: Measured continuously in the growth chamber
CONTROL AND BLANK SYSTEM
Inoculum blank with silica gel:18 bottles containing inoculum, mineral medium and silica gel
inoculum blank without silica gel: 10 bottles containing inoculum and mineral medium
procedure control: 10 bottles containing reference substance, inoculum and mineral medium
toxicity control: 6 bottles containing test item, reference substance, inoculum and mineral medium - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 2 mg/L
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 5
- Sampling time:
- 28 d
- Details on results:
- Test item was biodegraded by 5% at day 29 in the Closed Bottle test . The test period was therefore extended to 56 days in order to demonstrate a potential inherent biodegradability or a non persistence of the test item. However, the biodegradation percentage at day 56 was only 7%. It should be noted that the test item had no inhibitory effect on the activity of the microbial inoculum, as demonstrated by the test results of the toxicity control, in agreement with OECD 301 (oxygen consumption in the toxicity control must be greater than 25% of the ThOD value within 14 days).
Therefore, 2-ETHYL-4-(2,2,3-TRIMETHYL-3-CYCLOPENTENE-1-YL)-(2E)-BUTEN-1-OL cannot be considered as readily biodegradable under the experimental conditions. - Results with reference substance:
- The calculated ThOD of the test item were calculated from their empirical formulae to be ThOD = 3.00 mgO2.mg-1
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- 2-ETHYL-4-(2,2,3-TRIMETHYL-3-CYCLOPENTENE-1-YL)-(2E)-BUTEN-1-OL cannot be considered as readily biodegradable.
- Executive summary:
An enhanced biodegradability study was performed to assess the biotic degradation of test item 2-ETHYL-4-(2,2,3-TRIMETHYL-3-CYCLOPENTENE-1-YL)-(2E)-BUTEN-1-OL according to OECD Guideline 301 D and in compliance with GLP.
18 BOD bottles were filled with mineral medium inoculated with river water (5 mL/L) and test item added at a nominal concentration of 2 mg/L with the help of silica gel. In the meantime, two series of ten blanks (with and without silica gel) were filled with inoculated mineral medium. Furthermore, six bottles containing the reference substance sodium benzoate (2 mg/L) were tested in order to check the procedure. The concentrations of dissolved oxygen in duplicate bottles from each group incubated between 20.7 and 22.4°C in darkness were measured at the start of the test and thereafter once a week until the end of the test (56 days for each group except the procedure control until 14 days).
The validity of the test was demonstrated by an endogenous respiration < 1.5 mg O2/L and by a biodegradation of the reference substance of 84 % of its ThOD after 14 days of incubation. Moreover, the differences of the replicate values at day 56 were less than 20 % and oxygen concentrations were > 0.5 mg/L in all bottles during the test period. The test item was biodegraded by 5 % at day 29. Substances are considered to be readily biodegradable in the Closed Bottle test if oxygen consumption is equal to or greater than 60 % of the ThOD value within ten days from the day by which the level exceeding 10 % and this pass level is attained within 28 days. In this study, the pass level of 60 % was not reached.
Therefore, 2-ETHYL-4-(2,2,3-TRIMETHYL-3-CYCLOPENTENE-1-YL)-(2E)-BUTEN-1-OL cannot be considered as readily biodegradable.
Reference
Percentage biodegradation of the test item, the reference substance and the toxicity control throughout the test:
|
Time (days) |
|||||||||
0 |
7 |
14 |
21 |
29 |
35 |
42 |
49 |
56 |
||
Test Suspension (2 mg test item.L-1) |
Replicate 1 |
0.00 |
3.25 |
3.00 |
5.75 |
6.58 |
3.33 |
2.58 |
1.42 |
4.92 |
Replicate 2 |
0.00 |
3.25 |
4.67 |
1.75 |
4.25 |
5.17 |
0.75 |
4.75 |
9.25 |
|
Mean |
0 |
3 |
4 |
4 |
5 |
4 |
2 |
3 |
7 |
|
Procedure Control (2 mg reference substance.L-1) |
Replicate 1 |
0.00 |
79.49 |
84.88 |
69.46 |
88.17 |
- |
- |
- |
- |
Replicate 2 |
0.00 |
75.90 |
83.38 |
73.05 |
85.18 |
- |
- |
- |
- |
|
Mean |
0 |
78 |
84 |
71 |
87 |
- |
- |
- |
- |
|
Toxicity Control * (2 mg reference substance.L-1 + 2 mg test item.L-1) |
Mean |
0 |
71 |
78 |
- |
- |
- |
- |
- |
- |
* % biodegradationtox.Control= BOD tox. cont. x 100
ThODref. subst.
with BOD tox. cont = (mTox(o) - mTox(n)) - (ma(o) - ma(n))
mg test ref susbst.L-1in vessel
and where:
n = number of days after the start of the test
mTox(0) = mean toxicity control value at day 0
mTox (n) = mean toxicity control value at day n
ma(0) = mean test suspension value at day 0
ma(n) = mean test suspension value at day n.
Description of key information
An enhanced biodegradability study was performed to assess the biotic degradation of the test itemaccording to OECD Guideline 301 D and in compliance with GLP.
18 BOD bottles were filled with mineral medium inoculated with river water (5 mL/L) and test item added at a nominal concentration of 2 mg/L with the help of silica gel.
The test item was biodegraded by 5% at day 29.
It is therefore considered that the test substance is not readily biodegradable according to the criteria of OECD 301 D guideline.
The result of this key study is proposed as end point value for the chemical safety assessment.
2-ethyl-4-(2,2,3-trimethyl-3-cyclopenten-1-yl)-(2E)-buten-1-ol is not readily biodegradable according to the criteria of OECD 301D guideline.
The test item is not considered as readily biodegradable according to OECD 301 D guideline.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
An enhanced biodegradability study was performed to assess the biotic degradation of the test itemaccording to OECD Guideline 301 D and in compliance with GLP.
18 BOD bottles were filled with mineral medium inoculated with river water (5 mL/L) and test item added at a nominal concentration of 2 mg/L with the help of silica gel.
The test item was biodegraded by 5 % at day 29.
It is therefore considered that the test substance is not readily biodegradable according to the criteria of OECD 301 D guideline.
The result of this key study is proposed as end point value for the chemical safety assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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