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Diss Factsheets
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EC number: 203-322-1 | CAS number: 105-68-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reveiwed journal.
- Justification for type of information:
- Data is from peer reveiwed journal.
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- In order to discover the antimicrobial activity against skin resident microorganisms responsible for axillary odor, the minimum inhibitory concentrations (MICs) of common fragrance materials were determined by agar plate dilution method.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Name of test material (as cited in study report): Isoamyl propionate
Molecular formula (if other than submission substance): C8H16O2
Molecular weight (if other than submission substance): 144.212
Smiles notation (if other than submission substance): C(OCCC(C)C)(CC)=O
InChl (if other than submission substance):
1S/C8H16O2/c148(9)10657(2)3/h7H,46H2,13H3
Substance type: Organic
Physical state: Liquid - Analytical monitoring:
- yes
- Details on sampling:
- Sampling method: Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
Sample storage conditions before analysis: No data - Vehicle:
- yes
- Details on test solutions:
- Vehicle- Ethyl alcohol or dimethyl sulfoxide (DMSO).
- Test organisms (species):
- other: Corynebacterium minutissimum (CM) Arthrobacter sp, Staphylococcus aureus (IAM-1011, (SA)), Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC))
- Details on inoculum:
- Corynebacterium minutissimum (ATCC 23348, (CM)), was gifted from the Department of Dermatology, University of Pennsylvania.
Arthrobacter sp. isolated from Lipo-66 is a geophilic organism.
Staphylococcus aureus (IAM-1011, (SA)) was purchased from Institute of Applied Microbiology, Tokyo university
Staphylococcus epidermidis var. (SE) was gifted from the Department of Dermatology, University of Pennsylvania
Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university. - Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 24 h
- Remarks on exposure duration:
- No data available
- Post exposure observation period:
- No data available
- Hardness:
- No data available
- Test temperature:
- 37◦ C
- pH:
- 7.6-8
- Dissolved oxygen:
- No data available
- Salinity:
- No data available
- Conductivity:
- No data available
- Nominal and measured concentrations:
- No data available
- Details on test conditions:
- Test vessel: Agar plates
Material, size, headspace, fill volume:
Aeration: No data
Type of flow-through (e.g. peristaltic or proportional diluter): No data
Renewal rate of test solution (frequency/flow rate): No data
No. of organisms per vessel: microbial concentration of 106CFU/ml
No. of vessels per concentration (replicates):
No. of vessels per control (replicates): No data
No. of vessels per vehicle control (replicates): No data
Biomass loading rate: No data - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 24 h
- Dose descriptor:
- other: MIC
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Remarks on result:
- other: For all species
- Remarks:
- Non toxic
- Details on results:
- No data available
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- No data available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) and Arthrobacter species was >2000 mg/l (2000ppm) (inoculum 105CFU/plate) after 24 hours exposure to isoamyl propionate.
- Executive summary:
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) and Arthrobacter species was>2000 mg/l (2000ppm) (inoculum 105CFU/plate) after 24 hours exposure to isoamyl propionate.
Reference
Description of key information
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) and Arthrobacter species was >2000 mg/l (2000ppm) (inoculum 105CFU/plate) after 24 hours exposure to isoamyl propionate.
Key value for chemical safety assessment
Additional information
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) and Arthrobacter species was >2000 mg/l (2000ppm) (inoculum 105CFU/plate) after 24 hours exposure to isoamyl propionate.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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