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EC number: 227-105-6 | CAS number: 5657-17-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted in compliance with GLP regulations
- Justification for type of information:
- Study is read across and slightly updated and presented under "type of information" as experimental study until the lead company has updated the Source RSS
Read across justification is presented in IUCLID chapter 13 - Qualifier:
- according to guideline
- Guideline:
- other: EEC Guideline XI/681/86, Draft 4: "Prolonged toxicity study with Daphnia magna: Effects on reproduction"
- Principles of method if other than guideline:
- For the statistical evaluation of the LOEC and NOEC Duncan's new multiple range test was used.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for analysis were taken in the 1st, the 2nd and the 3rd week of the test. For each concentration the freshly prepared test solution (unstocked) and the corresponding 48 h or 72 h old test solution (stocked with daphnids) were analyzed.
- Vehicle:
- no
- Details on test solutions:
- At the beginning of the test and before changing the test solutions, a stock solution of the test substance was freshly prepared. A defined amount of the substance was weighed out and suspended directly in the test medium by stirring (about 10 min at 22+/-1 °C). The nominal concentrations of the stock solutions were 100 mg/L. By dilution of the stock solutions with test medium, the nominal test concentrations were prepared.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna STRAUSS
Supplier: Institut National de Recherche Chimique Appliquée, France, 1978
Culture: The daphnids are cultured under standard conditions in the Laboratory for Experimental Ecology of BASF AG, Ludwigshafen - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Hardness:
- Total hardness: 2.20 - 3.20 mmol/L
- Test temperature:
- 19.9 - 21.7 °C
- pH:
- 7.5-8.5
- Dissolved oxygen:
- Oxygen content: 8.0 - 9.6 mg/L
- Nominal and measured concentrations:
- Test concentrations (nominal): 100; 50.0; 25.0; 12.5; 6.25; 3.13 and 1.56 mg/L. (Dilution factor: 2)
- Details on test conditions:
- TEST PROCEDURE
- Semistatic procedure: Change of test solution three times a week.
- Test water: synthetic M4-medium
Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/L
Molar ratio Ca:Mg: about 4 : 1
Conductivity: 550 - 650 µS/cm
- Number of animals/vessel: 1
- Number of replicates/concentration: 10
- Number of replicates/control: 10
- Age of the animals at the start of the test: 2 - 24 h
- Age of stock animals: 2 - 4 weeks
- Light: Artificial light, OSRAM L58 W31 warm white day : night-rhythm = 16 : 8 hours
- Removal of young from test beakers and counting: daily
- Check of the study and recording (mortality, hatching of young): daily
- Feeding: daily
- Measurement of temperature: Continuously in an extra vessel
- Measurement of pH and oxygen: At the start of the test and at each change of test solution in the 48
- or 72-hour-old solution in one parallel at each concentration - Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 21 d
- Dose descriptor:
- other: LC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
Reference
Analytically found relative minimum and maximum concentrations of the test substance in the test solutions
at different times of the test (with and without daphnids). The values are given in percent of the nominal concentrations:
Time / Stocked with / Minimum / Maximum /
[h] / daphnids / [%] / [%] /
-----------------------------------------------------------
0 / no / 80.8 / 166.7 /
48/72 / no / 92.8 / 166.7 /
48/72 / yes / 95.2 / 173.1 /
-----------------------------------------------------------
M4-water caused a background value of about 1 mg/L "Trilon BD". Regarding to this and to the precision of the analytical method, most concentrations found corresponded to the expected values.
Validity criteria:
In the control:
- the mortality of parent animals up to the end of the test was <= 10 %.
- the mean number of living young per parent animal that survived the test was >= 60.
- the coefficient of variation for the mean number of living young per parent animal that survived the test was <= 25 %.
- The NOEC of the control substance 3,4-dichloroaniline was 2.5 ug/L, the LOEC was 10 ug/L and LC0 was > 10 ug/L.
The validity criteria were fulfilled by the test.
Control:
In the control the first young were observed at day 7. In the highest concentration tested, at which the daphnids produced young (100 mg/L), the first young were observed at day 13.
Description of key information
With high probability the test substance is chronically not harmful to aquatic invertebrates.
Key value for chemical safety assessment
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 21.7 mg/L
Additional information
No data on the long term toxicity to aquatic invertebrates is available for H2 ED2A. Consistent with the other ecotoxicity endpoints, the available data from H4 EDTA are read-across to fill the datagap. All ecotox and fate data from H4 EDTA, Na2H2 EDTA, Na4 EDTA, HEDTA, H2 ED2A etc have been collated in a document in which is compiled to justify the read across. This document is included in IUCLID 6, Chapter 13.
The long-term toxicity of EDTA to Daphnia magna was measured in an reproduction test according to GLP criteria [BASF AG, 1998]. In this test Na2H2 EDTA was used. In this test a NOEC of 21.7 mg/L (expressed as H4 EDTA) was observed after 21 days of exposure
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