Rhodium acetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 March 1986 – 26 March 1986

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted according to OECD guideline No. 401 and to GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Rhodium(3+) acetate

EC Number:

247-460-0

EC Name:

Rhodium(3+) acetate

Cas Number:

42204-14-8

Molecular formula:

C6H9O6Rh

IUPAC Name:

Rhodium(3+);triacetate

Test material form:

solid: crystalline

Details on test material:

- Name of test material (as cited in study report): rhodium (III) acetat “braun”
- Substance type: brown crystal
- Physical state: solid
- Composition of test material, percentage of components: 39.54% Rh
- Lot/batch No.: 6091
- Stability under test conditions: stable throughout the test period
- Storage condition of test material: at room temperature
- Other: soluble in water

Test animals

Species:

rat

Strain:

other: Wistar Bor: WISW

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Versuchstierzucht Winkelmann, D-4791 Borchen
- Age at study initiation: males 49-50 days, females 63-64 days
- Weight at study initiation: males 128-157 g, females 128-150 g
- Fasting period before study: 16 hrs
- Housing: individually
- Diet: ad libitum standard diet, ssniff R “special diet for rats” supplied by Firma ssniff Spezialfutter GmbH, D-4770 Soest
- Water: ad libitum
- Acclimation period: at least 5 days before dosing

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 55 ± 15
- Photoperiod (hrs dark / hrs light): 6 am-6 pm fluorescent tube lighting, 6 pm-6 am “natural light-dark-rhythm”

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 215 mg/ml
- Purity: demineralised

MAXIMUM DOSE VOLUME APPLIED: 31.6 ml/kg bw

Doses:

Reported as 2150, 3160, 4640 and 6810 mg/kg bw

No. of animals per sex per dose:

Groups of 5/sex/dose

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observed 6-8 hours after dosing then twice daily for 14 days or daily on Saturdays, Sundays and public holidays
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

Probit analysis

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Remarks on result:

other: Measured graphically

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 4 799 mg/kg bw

95% CL:

ca. 1 825 - ca. 22 444

Remarks on result:

other: Measured using Probit analysis

Mortality:

One of the five females given 4640 mg/kg bw died within days [although a summary table suggests that two died within 2-6 days]. At the top dose, 4 of the 5 males died after 330-370 mins or 5-6 days and all five females died after 140-390 mins or 2 days.

Clinical signs:

other: No effects were seen in either the males or females at 2150 or 3160 mg/kg bw. Cyanosis was evident in 2 males and 3 females at 4640 mg/kg bw, and in 2 males and 1 female at 6810 mg/kg bw. The top dose also caused a loss of co-ordination in 2 males and 4 f

Gross pathology:

The intestinal mucous membranes were red or yellow in some of the animals.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute oral median lethal dose (LD50) of rhodium (III) acetate “brown” was found to be >5000 and 4799 mg/kg bw in male and female rats, respectively.

Executive summary:

The acute oral toxicity of rhodium (III) acetate “brown” was investigated in an OECD Test Guideline 401 study, conducted according to GLP. The test substance was administered by oral stomach tube to rats (5/sex/dose) at 2150, 3160, 4640 or 6810 mg/kg bw and animals were observed for 14 days. Deaths occurred at the top dose in all but one male animal, and in one female at 4640 mg/kg bw. Cyanosis (a blue discolouration of the skin and mucous membranes) was evident from 4640 mg/kg bw, and there were various effects on the nervous system at the top dose.

 

The oral LD50 value was determined to be >5000 mg/kg bw in males (measured graphically) and 4799 mg/kg bw in females (calculated by probit analysis).

 

Based on the results of this study, no classification is required for acute oral toxicity according to EU CLP criteria (EC 1272/2008).