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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-reference
Reason / purpose:
other: Reference to same publication
Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Reason / purpose:
other: reference to the same publication
Related information:
Composition 1
Reference:
Composition 1
Principles of method if other than guideline:
No data
GLP compliance:
not specified
Test material information:
Composition 1
Analytical monitoring:
no
Vehicle:
not specified
Details on test solutions:
Test solutions were prepared in well water having a total alkalinity of 138 mg/L. A photoperiod of 16h light/8h dark was maintained.
Test organisms (species):
Daphnia magna
Details on test organisms:
The daphnia were less than 24 h old.
Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h
Hardness:
192 mg/L
Test temperature:
20°C ± 1°C
pH:
8.2
Nominal and measured concentrations:
No data
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 12
Daphnia tests were performed in 205-mL beakers containing 200 mL of test solution.

The number of survivors in each test concentrations was monitored daily (swimming ability was the criterion for survival).
The 48h-EC50 (median effective concentration, i.e., concentration which immobilized 50% of the exposed animals) was calculated by means of the method of Litchfield and Wilcoxon.
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2 000 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No data
Reported statistics and error estimates:
No data
Validity criteria fulfilled:
not specified
Conclusions:
The 48-hour EC50 was 2000 mg/L. Trichloroacetic acid is not harmful to daphnia.
Executive summary:

The objective of the study was to determine the acute toxicity of Trichloroacetic acid (TCA) to Daphnia magna.

The 48 -hour EC50 was determined to be 2000 mg/L.

In these test conditions, TCA is not harmful to daphnia.

Data source

Reference
Reference Type:
publication
Title:
Degradation of Diazinon by Sodium Hypochlorite. Chemistry and Aquatic Toxicity
Author:
William H. Dennis, Jr., Eugene P. Meier, William F. Randall, Alan B. Rosencrance and David H. Rosenblatt
Year:
1979
Bibliographic source:
Environmental Science & Technology, Volume 13, Number 5, May 1979, 594-598

Materials and methods

Principles of method if other than guideline:
No data
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
No data available

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
not specified
Details on test solutions:
Test solutions were prepared in well water having a total alkalinity of 138 mg/L. A photoperiod of 16h light/8h dark was maintained.

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
The fish were less than 1 year old.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
192 mg/L
Test temperature:
20°C ± 1°C
pH:
8.2
Nominal and measured concentrations:
No data
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
Fish tests were conducted in 19-L jars containing 14 L of test solution.

The number of survivors in each test concentrations was monitored daily.
The 96h-LC50 (median lethal concentration, i.e., concentration lethal to 50% of the exposed animals) was calculated by means of the method of Litchfield and Wilcoxon.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2 000 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No data
Reported statistics and error estimates:
No data

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Under the test conditions, the 96-hour LC50 was 2000 mg/L. Trichloroacetic acid is not harmful to fish.
Executive summary:

The objective of the study was to determine the acute toxicity of Trichloroacetic acid (TCA) to Pimephales promelas.

The 96 -hour LC50 was determined to be 2000 mg/L.

In these test conditions, TCA is not harmful to fish.