Reaction mass of 1-amino-9,10-dioxo-4-(phenylamino)-9,10-dihydroanthracene-2-sulfonic acid and sodium 1-amino-9,10-dioxo-4-phenylaminoanthracene-2-sulphonate

Short-term toxicity to aquatic invertebrates

Description of key information

EC50 > 67mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

67 mg/L

Additional information

No studies on the "Short-term toxicity to aquatic invertebrates" are available for the substance in itself.

Nevertheless a study has been conducted with an analogue molecule (Similar Substance 02). Further information are reported in the Read Across justification attached to section 13.

The short term toxicity to aquatic invertebrates was determined using the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).

Although the test substance is soluble in pure (deionised) water ( about 80 g/l), most of the test substance in the stock solution settled down and build a sediment at the bottom of the flask. This effect was possibly caused by a reaction of the test substance with the water hardness compounds in the test water. For the preparation of the test concentrations only the supernatant of the stock solution was taken ( limit of the solubility ). The reported results are related to the actual mean values of the analysed conentrations of the test article measured at the beginning and at the end of the test. No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC value after 48 hours was determined to be 34 mg/l, the EC50 and the EC 100 value could not be calculated, but are clearly higher than 67 mg/l.