Ethyl 3,5,5-trimethylhexanoate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, part 27

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 3,5,5-trimethylhexanoate

EC Number:

266-959-4

EC Name:

Ethyl 3,5,5-trimethylhexanoate

Cas Number:

67707-75-9

Molecular formula:

C11H22O2

IUPAC Name:

ethyl 3,5,5-trimethylhexanoate

Details on test material:

- Name of test material (as cited in study report): 3,5,5-Trimethyl-ethyl-capronat
- Physical state: liquid
- Analytical purity: >95% a.i.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- preparation of stock solution (10 g/100 mL), treated with ultraturrax for 10 min
- volume required for preparation of test solutions was taken while continuously stirring the stock solution

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Pseudomonas putida HIGULA, strain: Berlin 33/2 (DSM 50026)
- age of suspension at the start of the test: 1 d

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Hardness:

no data

Test temperature:

no data

pH:

no data

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

300, 1000, 3000, 10000 mg/L

Details on test conditions:

TEST SYSTEM
- fill volume: 100 mL
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 5


TEST MEDIUM / WATER PARAMETERS
- H5 - water

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Key result

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

1 200 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

no data

Results with reference substance (positive control):

n.a.

Any other information on results incl. tables

	mg/L O2/min	Inhibition [%]
Control	2.15	n.a.
Test substance, 10000 mg/L	1.55	28
Test substance, 3000 mg/L	1.80	16
Test substance, 1000 mg/L	1.95	9
Test substance, 300 mg/L	1.95	9

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The 30 min EC0 of 3,5,5-Trimethyl-ethyl capronate to Pseudomonas putida was 1000 mg/L, the 30 min EC10 was 1200 mg/L.

Executive summary:

In a 30 min Pseudomonas oxygen consumption inhibition test according to DIN 38412 part 27, Pseudomonas putida was exposed to 3,5,5-Trimethyl-ethyl capronate (95% a.i.) at nominal concentrations of 0 (control), 300, 1000, 3000 and 10000 mg/L.

Oxygen consumption was reduced by 28% at 10000 mg/L compared to the control, by 16% at 3000 mg/L and by 9% at 1000 and 300 mg/L.

The 30 min EC0 of 3,5,5-Trimethyl-ethyl capronate to Pseudomonas putida was 1000 mg/L, the 30 min EC10 was 1200 mg/L.