2,2,6,6-tetramethyl-4-piperidylamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,2,6,6-tetramethyl-4-piperidylamine

EC Number:

253-197-2

EC Name:

2,2,6,6-tetramethyl-4-piperidylamine

Cas Number:

36768-62-4

Molecular formula:

C9H20N2

IUPAC Name:

2,2,6,6-tetramethylpiperidin-4-amine

Constituent 2

Reference substance name:

Triacetondiamine

IUPAC Name:

Triacetondiamine

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

During this study samples of the test substance concentrations of nominal 6.25; 25; 100 mg/L M4 medium and the control (M4 medium) were taken after 0 h, 24 h and 48 h. Analytical determination showed no loss of test substance during the exposure period of 48 h. Recovery of test substance was between 104% and 118% of nominal test concentration.

Test solutions

Vehicle:

no

Details on test solutions:

The test concentrations were prepared by diluting a stock solution of 1 g/L. 200 mg test substance was weighed into a beaker, mixed with M4 medium and was stirred for 5 min. with a magnetic stirrer to dissolve the test substance in the test medium. Then the test substance preparation was filled into a graduated flask and made up to a total volume of 200 mL with M4 medium. The amounts corresponding to the test concentrations were pipetted into a graduated flask, made up to a total volume of 500 mL with M4 medium, and then transferred to the test vessels.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test organisms were <24 hours old and bred at the testing facility. The daphnids were kept in M4 medium in a 16:8 hours light-dark cycle at a temperature of 20 +/- 2°C and were fed with singular algae.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 +/- 2°C

pH:

tests start:8.4 - 9.6
test end: 8.4 - 8.5

Dissolved oxygen:

test start: 5.8 - 6.2
test end: 5.6 - 6.0

Salinity:

no data

Nominal and measured concentrations:

nominal: 6.25, 12.5, 25.0, 50.0 and 100 mg/L
Recovery of the test substance at test end (48h) was between 104% and 118% of nominal values.

Details on test conditions:

according to guideline

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

45.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL 37.9-55.6

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (24h): 0.8 mg/l

Reported statistics and error estimates:

EC50 values, 95% confidence limits and NOEC were calculated with the ToxRat Professional software.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under presen conditions tested , a IC50 value of the test substance after 48h exposure of Daphnia magna is derived to be 45.9 mg/L (nominal). The test substance is stable in aqueous solution (recovery rates 104-118% of nominal).