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EC number: 204-980-2 | CAS number: 130-20-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 12 March 1974
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- No information given on results with negative control.
- Qualifier:
- according to guideline
- Guideline:
- other: The test was conducted according to SOP B-10 (1977-09-01).
- Principles of method if other than guideline:
- - Principle of test: Determination of an acute bacteriostatic toxicity to activated sludge und aerobic conditions.
- Short description of test conditions: Bacteria suspension from activated sludge filtrate is incubated for 12 - 24 h at 25°C in suspension tubes with nutrient broth (OECD).
- Parameters analysed / observed: Growth inhibition is determined visually (turbidity). The toxicity to activated sludge is determined as the lowest effect concentration where no growth is observed compared to the negative control. - GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution is diluted to 1, 5, 10, 20, 50, 100, 200 and 300 ppm dilutions. 0.1 mL of each dilution is mixed with 4.5 mL nutrient broth in a test tube.
- Controls: negative control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (<2%) or dimethyl formamide (<1%) if not water soluble - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: yes
- Method of cultivation: activated sludge bred in a aerated Husmann apparatus, weekly preparation
- Preparation of inoculum for exposure: 100 mL activated sludge from day 3 to 5 are taken from the effluent and filtrated with Whatman GF/A. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 25 +/- 2°C
- Nominal and measured concentrations:
- Nominal: 1, 5, 10, 20, 50, 100, 200, 300 ppm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: tube
- Material, size, headspace, fill volume: glass
- Nutrients provided for bacteria: nutrient broth (OECD)
- Test tubes provided with nutrient broth and the respective test substance concentration are inoculated with 1 drop of filtrated bacteria suspension from activated sludge. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48-h ErC0 for bacteria from activated sludge was determined to be >300 mg/L.
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 28 November 1977
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- No information given on results with negative control.
- Qualifier:
- no guideline available
- Version / remarks:
- The test was conducted according to SOP B-10 (1977-09-01).
- Principles of method if other than guideline:
- - Principle of test: Determination of an acute bacteriostatic toxicity to activated sludge und aerobic conditions.
- Short description of test conditions: Bacteria suspension from activated sludge filtrate is incubated for 12 - 24 h at 25°C in suspension tubes with nutrient broth (OECD).
- Parameters analysed / observed: Growth inhibition is determined visually (turbidity). The toxicity to activated sludge is determined as the lowest effect concentration where no growth is observed compared to the negative control. - GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution is diluted to 1, 5, 10, 20, 50, 100, 200 and 300 ppm dilutions. 0.1 mL of each dilution is mixed with 4.5 mL nutrient broth in a test tube.
- Controls: negative control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (<2%) or dimethyl formamide (<1%) if not water soluble - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: yes
- Method of cultivation: activated sludge bred in a aerated Husmann apparatus, weekly preparation
- Preparation of inoculum for exposure: 100 mL activated sludge from day 3 to 5 are taken from the effluent and filtrated with Whatman GF/A. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 25 +/- 2°C
- Nominal and measured concentrations:
- Nominal: 1, 5, 10, 20, 50, 100, 200, 300 ppm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: tube
- Material, size, headspace, fill volume: glass
- Nutrients provided for bacteria: nutrient broth (OECD)
- Test tubes provided with nutrient broth and the respective test substance concentration are inoculated with 1 drop of filtrated bacteria suspension from activated sludge. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48-h ErC0 for bacteria from activated sludge was determined to be >300 mg/L.
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 24 May 1978
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- No information given on results with negative control.
- Qualifier:
- no guideline available
- Version / remarks:
- The test was conducted according to SOP B-10 (1977-09-01).
- Principles of method if other than guideline:
- - Principle of test: Determination of an acute bacteriostatic toxicity to activated sludge und aerobic conditions.
- Short description of test conditions: Bacteria suspension from activated sludge filtrate is incubated for 12 - 24 h at 25°C in suspension tubes with nutrient broth (OECD).
- Parameters analysed / observed: Growth inhibition is determined visually (turbidity). The toxicity to activated sludge is determined as the lowest effect concentration where no growth is observed compared to the negative control. - GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution is diluted to 1, 5, 10, 20, 50, 100, 200 and 300 ppm dilutions. 0.1 mL of each dilution is mixed with 4.5 mL nutrient broth in a test tube.
- Controls: negative control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (<2%) or dimethyl formamide (<1%) if not water soluble - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: yes
- Method of cultivation: activated sludge bred in a aerated Husmann apparatus, weekly preparation
- Preparation of inoculum for exposure: 100 mL activated sludge from day 3 to 5 are taken from the effluent and filtrated with Whatman GF/A. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Nominal and measured concentrations:
- Nominal: 1, 5, 10, 20, 50, 100, 200, 300 ppm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: tube
- Material, size, headspace, fill volume: glass
- Nutrients provided for bacteria: nutrient broth (OECD)
- Test tubes provided with nutrient broth and the respective test substance concentration are inoculated with 1 drop of filtrated bacteria suspension from activated sludge. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48-h ErC0 for bacteria from activated sludge was determined to be >300 mg/L.
Referenceopen allclose all
Description of key information
Three 48 -h ErC0s were determined to be >300 mg/L for bacteria suspensions from activated sludge. No toxic effect to bacteria was observed up to the water solubility limit (< 1.0 mg/L) based on a weight-of-evidence approach.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.