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EC number: 457-690-5 | CAS number: 23432-65-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 Aug 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 1984
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Energie, Landwirtschaft und Forsten, Wiesbaden, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Since the test item hydrolyses in water very quickly, the hydrolysis products were tested, according to the draft guidance document on aquatic toxicity testing on difficult substances. Therefore, 1000 mg test item were filled up to 200 mL with deionized water and were completely dissolved by stirring for 24 h. Thus a concentration of 5 g/L was obtained. Then, adequate volumes of the intensively stirred stock solution were dosed into the test flasks.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source of inoculum: Sewage treatment plant Groß-Zimmern, Germany
- Laboratory culture: no
- Preparation of inoculum for exposure: The sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. The latter procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (g) to its dry weight (g) determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 2.5 g dry material per litre, were made up with tap water. The activated sludge was used of the test directly after conditioning. The pH of the sludge was 7.4. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21 - 22 °C
- pH:
- 7.2 - 8.3
- Dissolved oxygen:
- 7.6 - 8.1 (start)
7.0 - 7.5 (end) - Nominal and measured concentrations:
- nominal: control, 10, 32, 100, 320, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks of approx. 1 L volume and Karlsruher flasks of 250 mL volume
- Aeration: with compressed air (approx. 0.6 L/min)
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED
Respiration rate was measured in Karlsruher flasks after exactly 3 h with an oxygen electrode and was recorded for about 10 min. During measurement the samples were continuously stirred on a magnetic stirrer. The O2 consumption was determined from the most linear part of the respiration curve.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.125 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but the exposure was to hydrolysis products
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but the exposure was to hydrolysis products
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC50 (3 h): 6.9 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- An 3 h EC50 > 1000 mg/L has been determined for the effects of the test substance on respiration rate of activated sludge microorganisms. The results are expressed relative to nominal concentrations of the test substance. However, the substance is subject to rapid hydrolysis and under the test conditions it is therefore likely that exposure will have been to its hydrolysis products.
Reference
Table 2: Inhibition of respiration after 3 h
Flask |
Concentration [mg/L] |
Oxygen consumption [mg O2/L min] |
Inhibition [%] |
5 |
1000 |
0.330 |
10.8 |
6 |
320 |
0.380 |
-2.7 |
7 |
100 |
0.400 |
-8.1 |
8 |
32 |
0.400 |
-8.1 |
9 |
10 |
0.390 |
-5.4 |
1 |
control |
0.350 |
-- |
10 |
control |
0.390 |
-- |
Description of key information
EC50 (3 h): > 1000 mg/L based on respiration of activated sludge microorganisms (OECD 209)
Key value for chemical safety assessment
Additional information
One experimental study is available investigating the toxicity of methyl-N-{[dimethoxy(methyl)silyl]methyl}carbamate (CAS 23432-65-7) to activated sludge microorganisms (Hertl, 2003). This study was performed according to OECD 209 (GLP). Activated sludge from a domestic sewage treatment plant was exposed to nominal concentrations of 10, 32, 100, 320 and 1000 mg/l. Since the substance is expected to hydrolyse rapidly in contact with water (DT50: 1.9 h at 20 - 25 °C, pH 7), the result from this study mainly investigated the possible effects of the hydrolysis products (Methyl-N-{[dihydroxy(methyl)silyl]methyl}carbamate und methanol). During stock solution preparation it was ensured by intensive stirring that the parent substance hydrolyses and mainly the hydrolysis products are tested. After an exposure of 3 h an inhibition of respiration of 10.8% was recorded in the highest concentration tested, resulting in an EC50 > 1000 mg/l.
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