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EC number: 211-448-3 | CAS number: 645-62-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restriction (e.g. non-GLP, no analytical verification of test item concentrations)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: German standard DIN 38 412 L15
- GLP compliance:
- no
Test material
- Reference substance name:
- 2-ethylhex-2-enal
- EC Number:
- 211-448-3
- EC Name:
- 2-ethylhex-2-enal
- Cas Number:
- 645-62-5
- Molecular formula:
- C8H14O
- IUPAC Name:
- 2-ethylhex-2-enal
- Details on test material:
- - Name of test material (as cited in study report): 2-Ethylhexen-2-al crude
- Analytical purity: >= 95 %)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria. No undissolved test substance was visible.
Test organisms
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden orfe
- Length at study initiation (length definition, mean, range and SD): 6.7 cm (range: 6.0-7.2 cm)
- Weight at study initiation (mean and range, SD): 2.8 g (range: 2.0-3.6 g)
- Feeding during test: 1 day before and during exposure
ACCLIMATION
- Acclimation period: 3 days
Medical treatment during housing: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 2.5 mmol/l
- Test temperature:
- 20 °C
- pH:
- 7.4 - 7.8
- Dissolved oxygen:
- 8.1 - 8.8 mg/l
- Nominal and measured concentrations:
- Nominal test concentrations: 0 (control), 4.64, 10.0, 21.5, 46.4, 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: non-sealed all-glass aquaria (30x22x24 cm)
- Fill volume: 10 l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.8 g fish / l test water
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater was prepared from fully demineralized tap water according to DIN 38 412, part 11 (Oct 1982) that was resalted by the addition of 294.0 mg/l CaCl2*2H2O, 123.3 mg/l MgSO4*7H2O, 63.0 mg/l NaHCO3 and 5.5 mg/l KCl.
- Acid capacity: 0.8 mmol/l
- Na/K ions: 10:1
- Ca/mg ratio: 4:1
- Intervals of water quality measurement: measurements: temperature, pH, and oxygen content after 1, 24, 48, 72, and 96 h
OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms after 1, 4, 24, 48, 72, and 96 h
- Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: symptoms of intoxication
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 14.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 21.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- LC50 (48 h): 32 mg/l (this lethal concentration corresponds to the normal sensitivity)
- Reported statistics and error estimates:
- Probit analysis (Finney 1971). The LC50 value was calculated as the geometrical mean of the 10.0 mg/l and the 21.5 mg/l value.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
- Mortality:
Nominal test Cumulative mortality (n) after x hours
concentration 1 4 24 48 72 96
(mg/l)
0 (control) 0 0 0 0 0 0
4.64 0 0 0 0 0 0
10.0 0 1 1 1 1 1
21.5 0 10 10 10 10 10
46.4 0 10 10 10 10 10
100.0 0 10 10 10 10 10
Symptoms of intoxication:
Nominal test Symptoms of intoxication after x hours
concentration 1 4 24 48 72 96
(mg/l)
0 (control) - - - - - -
4.64 - - - - - -
10.0 - - - - - -
21.5 NT + + + + +
46.4 + + + + + +
100.0 + + + + + +
Definitions of Symptoms:
-: No symptoms
+: All fish dead
N: Narcotic-like state
T: Tumbling
pH:
Nominal test pH after x hours
concentration 1 4 24 48 72 96
(mg/l)
0 (control) 7.5 7.7 7.7 7.8 7.7
4.64 7.4 7.5 7.6 7.5 7.6
10.0 7.4 7.4 7.5 7.5 7.5
21.5 7.4
46.4 7.4
100.0 7.4
Dissolved oxygen:
Nominal test dissolved oxygen (mg/l) after x hours
concentration 1 4 24 48 72 96
(mg/l)
0 (control) 8.1 8.3 8.6 8.1 8.2
4.64 8.1 8.5 8.8 8.4 8.2
10.0 8.3 8.8 8.5 8.4 8.2
21.5 8.5
46.4 8.6
100.0 8.7
Temperature:
The temperature remained constantly at 20 °C in all test vessels throughout the test.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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