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EC number: 944-232-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5 February 1993 to 22 February 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- The concentration of the test substance in the stock solution is 0.1 g/L in water, instead 0.5 g/L.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- Not performed
- Vehicle:
- no
- Details on test solutions:
- Not specified
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Organism: Activated sludge of a communal sewage treatment plant (ARA-Pro Rheno Basel)Sludge pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period specified
- Hardness:
- No data
- Test temperature:
- 20.4°C
- pH:
- 6.4 before the test beginning
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal: 64, 20 and 6.4 mg/L of the test substance
Nominal: 32,10 and 3.2 mg/L of the reference substance - Details on test conditions:
- Temperature: 20.4°C
Sludge dry weight: 1.56 g/L
Test volume: 100 mL
Test duration: 3h
Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode system: OXI DIGI 530 (SAV : 00 109)
Aeration: 0.1 - 0.2 L/min by pasteur-pipette
pH of sludge: 6.4 before the test beginning - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Substance is miscible in water -> dispersion
- Results with reference substance (positive control):
- The IC50 of the reference substance 3,5-Dichlorophenol : 8.0 mg/l.
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 (3 h) of the test material : > 64.0 mg/l.
- Executive summary:
The toxicity of the test material to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline No. 209, and performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.
The test substance concentrations tested were 64, 20 and 6.4 mg/l. The reference substance 3,5-Dichlorophenol was tested in concentrations of 32,10 and 3.2 mg/l.
Results:
IC50 of the test material: > 64.0 mg/l
IC50 of the reference substance: 8.0 mg/l
Reference
Control |
pH after 3 h |
1) O2-consumption (in cm) |
2) time (in cm) |
mg O2/min/l |
|
C1 C1 |
7.6 7.5 |
4.2 4.2 |
3.8 4.1 |
0.88 0.82 |
Φ C 0.85 |
Test substance (mg/l) |
pH after 3 h |
1) O2-consumption (in cm) |
2) time (in cm) |
mg O2 pro min/l |
% of control |
% inhibition |
64 |
7.3 |
4.9 |
4.4 |
0.89 |
104.71 |
0.00 |
20 |
7.5 |
4.5 |
4.2 |
0.86 |
101.18 |
0.00 |
6.4 |
7.6 |
4.3 |
4.1 |
0.84 |
98.62 |
1.18 |
3,5-Dichlorophenol (mg/l) |
pH after 3 h |
1) O2-consumption (in cm) |
2) time (in cm) |
mg O2 pro min/l |
% of control |
% inhibition |
32 |
7.7 |
0.8 |
5.3 |
0.12 |
14.12 |
85.88 |
10 |
7.7 |
2.3 |
4.8 |
0.38 |
44.71 |
55.29 |
3.2 |
7.7 |
3.9 |
5.2 |
0.60 |
70.59 |
29.41 |
C = Control
1)2 cm = 0.8 mg/l O2
2)2 cm = 1 min
Remark: Substance is miscible in water → dispersion
Description of key information
Key value determined in a GLP accredited laboratory study performed in accordance with OECD Guideline No. 209.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 64 mg/L
Additional information
The toxicity of the substance to activated sludge was evaluated in a GLP study conducted in accordance with OECD Guideline No. 209. The substance concentrations tested were 64, 20 and 6.4 mg/L. The IC50 of the test material was determined to be > 64.0 mg/L.
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