Registration Dossier
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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test substance was tested for ready biaodegradability according to OECD guideline No. 301 B: "Ready Biodegradability: CO2 Evolution Test" adopted July 17, 1992, and the Commission Regulation (EC) No. 440/2008 of 30 May 2008, Part C.4-C: Carbon dioxide (CO2) evolution test (Modified Sturm Test) as well as for inherent biodegradation according to OECD Guideline 302 B: Inherent biodegradability: Zahn-Wellens/EMPA Test (July 17, 1992).
The test substance was not readily and not inherently biodegradable under test conditions.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Lithium salt of branched-aliphatic dicarboxylic acid was evaluated in a readily and an inherently biodegradation test.
For readily biodegradation, the test substance was tested in duplicate at 21.5 mg/L, corresponding to 12 mg TOC/L. The study consisted of six bottles: 2 inoculum blanks (no test substance), 2 test bottles (Lithium salt of branched-aliphatic dicarboxylic acid), 1 positive control (sodium acetate) and 1 toxicity control (Lithium salt of branched-aliphatic dicarboxylic acid plus sodium acetate). Test duration was 28 days (last CO2 measurement on day 29).
The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of the test substance. In the toxicity control,
Lithium salt of branched-aliphatic dicarboxylic acid was found not to inhibit microbial activity.
The inherent biodegradability of the test item was evaluated in an aerobic static test by determination of Dissolved Organic Carbon (DOC) removal. The test substance was analysed in duplicate at a nominal concentration of 51.5 mg/L and 51.2 mg/L as DOC using activated sludge at a concentration of suspended solids of 200 mg dry matter/L in the final inoculated mixture. Test duration was 28 days.
The degradation of diethylene glycol (as reference item) showed, that the test was considered to be valid. Due to the results of the toxicity control test, containing both the test substance and a reference substance, the test substance can be assumed to be not inhibitory.
The averaged percentage removal of DOC in the test suspension was 8.8%. In conclusion, the test item did not demonstrate inherent biodegradability.
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