2-Propyl-4-methyl-6-(1-methylbenzimidazol-2-yl)benzimidazole

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009-08-04 to 2009-08-18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study according to guideline OECD 207 .

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-Propyl-4-methyl-6-(1-methylbenzimidazole-2-yl)benzimidazole

IUPAC Name:

2-Propyl-4-methyl-6-(1-methylbenzimidazole-2-yl)benzimidazole

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Storage condition of test material: storage room of the institute, preservation condition normal temperature

Sampling and analysis

Analytical monitoring:

not specified

Test substrate

Vehicle:

yes

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): test substance was dissolved in 2 ml methanol (concentration not specified) and mixed with fine quarz sand. After evaporation the mixture was mixed with the basic test substate.- Controls: yes- Chemical name of vehicle (organic solvent, emulsifier or dispersant): methanol- Concentration of vehicle in test medium (stock solution and final test solution): not specified- Evaporation of vehicle before use: yes

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM- Source: Shanghai Yinquan Agriculture develpment Co., Ltd (ID: EF-MKT-090511)- Age at test initiation (mean and range, SD): five month old adult earthworms from the same population- Weight at test initiation (mean and range, SD): 300 to 600 mgACCLIMATION- Acclimation period: at least 14 days quarantied prior to use in test, 7 days bred in cow dung soil prior to test- Acclimation conditions (same as test or not): 20+-2°C

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

20-21°C

pH:

7.12, determined at the test start

Moisture:

32-35%

Details on test conditions:

TEST SYSTEM- Test container (material, size): 1L glas beaker - Amount of soil or substrate: 750 g test substrat- No. of organisms per container (treatment): 10- No. of replicates per treatment group: 4- No. of replicates per control: 4SOURCE AND PROPERTIES OF SUBSTRATE (if soil)Artificial soil test substrate was mixid by using 10 per cent sphagnum peat (pH 5.72, no visible plant remains, finely ground, moisture content of 3%)20 per cent kaolinite clay 70 per cent silica sand (particles of 0.2 mm diameter or lessMoisture content was adjusted with deionizid water or test solution to be 35 %OTHER TEST CONDITIONS- Photoperiod: 24 hours light- Temperature : 20°C to 21°CEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :Lethal effects at 7 and 14 days,: weight and numbers of earthworms were determined at the begining and the end of the testAny adverse effect was recordedTemperature and moisture were measured at the beginning and the end of the testpH was measured at the beginning of the testTEST CONCENTRATIONS- Justification for using less concentrations than requested by guideline: based on the results of the range finding tests , the 14 d LC 50 was expected to be higher than 1000 mg/kg- Range finding study: yes, - Test concentrations: 10, 100, 1000 mg/kg dry artificial soil weight, highest concentration causes no mortality.- Results used to determine the conditions for the definitive study: yes

Nominal and measured concentrations:

1000 mg/kg dry artificial soil weight (nominal)

Reference substance (positive control):

yes

Remarks:

chloracetamide (not determined during the test but in the same year 20 February to 6 March 2009)

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: no mortality- Total mass of adults at beginning of test: 310 to 330 mg- Total mass of adults at end of exposure period: 300 to 390 mg; Significant changes of the body weight (p>0.05) of the surviving earthworms were not observed at the end of the test- Morphological abnormalities: none- Behavioural abnormalities: none

Results with reference substance (positive control):

- Results with reference substance valid?: yesReference substance chloracetamide was tested at 4, 8, 16, 31, 60 mg/kg dry artificial soil. The 14 day-LC50 of a reference substance was determined to be 10 mg/kg dry soil weight (95% CL = 6 to 8 mg/kg dry soil weight) assuring that the laboratory test conditions were adequate.

Any other information on results incl. tables

Mortability of Eisenia foetida exposed to the test substance at 7 and 14 days

	Controls 0 mg/kg dry soil weight		Treatment 1000 mg/kg dry soil weight
Replicates	7 days	14 days	7 days	14 days
A	0	0	0	0
B	0	0	0	0
C	0	0	0	0
D	0	0	0	0
Total number of earthworms	40	40	40	40
Number of death	0	0	0	0
Mortality (%)	0	0	0	0

 No particular behavioral and morphological changes were observed.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the results, the 7 day and 14 day LC 50 was >1000 mg/kg dry soil weight