Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
other: no GLP + only for 24h exposure
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
ISO 6341 15 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Version / remarks:
edited in May 1996
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Remarks:
concentration of cetoxenone of the aqueous solution has been determined from carbon rate measured by carbon analyzer Shimadzu TOC 5050

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The daphnies used are derived from a farm made from eggs from the toxicity screening test kit "Daphtoxkit F Magna".

Study design

Test type:
not specified
Water media type:
other: mineral water suspension
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
Visual observation after 24h of the immobilisation of Daphnies.

Test conditions

Hardness:
Not specified
Test temperature:
Ambient temperature
pH:
Not specified
Dissolved oxygen:
Not specified
Nominal and measured concentrations:
Nominal: 6.8, 3.4, 1.7, 0.85, 0.43, 0 mg/L
Measured: -
Details on test conditions:
Ambiant temperature, protection from light
Assays are made in 4 replicates
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Key result
Duration:
24 h
Dose descriptor:
other: nominal concentration inducing 5% daphnies immobilisation
Effect conc.:
ca. 6.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The most concentrated aqueous solution of cetoxetone (6.8 mg/L) triggers an immobilisation of 5% of daphnies. Cetoxenone is thus not toxic to daphnies.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the experimental results, a saturated aqueous solution of cetoxenone at 6.8 mg/L is not toxic to daphnies.