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EC number: 231-218-6 | CAS number: 7450-69-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-11-12 - 2012-12-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to an internationally accepted guideline. All study parameters are based on the specific guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Guideline:
- other: Human Cornea Model Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Phenyl diamidophosphate
- EC Number:
- 231-218-6
- EC Name:
- Phenyl diamidophosphate
- Cas Number:
- 7450-69-3
- Molecular formula:
- C6H9N2O2P
- IUPAC Name:
- [(diaminophosphoryl)oxy]benzene
- Test material form:
- solid: crystalline
- Details on test material:
- Identity: PPDA
CAS-No.: 7450-69-3
EINECS-No.: 231-218-6
Molecular Formula: C6H9N2O2P
Purity: 92.4% (HPLC)
Constituent 1
Test animals / tissue source
- Species:
- other: Human Cornea Model
Test system
- Vehicle:
- water
- Amount / concentration applied:
- Each about 100 mg of the solid test item was applied to each of duplicate tissues, wetted with 30 µL of deionised water, and spread to cover the surface of the tissues evenly.
For the positive and negative controls 100 µL were dosed per tissue. - Duration of treatment / exposure:
- 3, 30 and 60 min.
Results and discussion
In vitro
Results
- Irritation parameter:
- other: relative absorbance (%of negative control)
- Run / experiment:
- test item
- Value:
- 16.1
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Interpretation of results:
- moderately irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- In this study and under the experimental conditions reported, the test item PPDA possesses a moderate eye irritating potential.
- Executive summary:
This in vitro study was performed to assess the eye irritation potential of PPDA by means of the Human Cornea Model Test.
Each about 100 mg of the solid test item was applied to each of duplicate tissues, wetted with 30 µL of deionised water, and spread to cover the surface of the tissues evenly.
100 µL of either the negative control (deionised water) or the positive control (0.3% Triton X-100) were applied to each tissue.
Tissues of the human cornea model EpiOcular™were treated with the test item for 3, 30 and 60 minutes in duplicate. The cells for the negative control were treated for 60 min and the positive control for 15 and 45 min each in duplicate.
After treatment with the negative control the absorbance values were well above the required acceptability criterion of mean OD≥0.8 for the60 minutestreatment interval thus showing the quality of the tissues.
Treatment with the positive control induced a decrease in the relative absorbance compared with the negative control to 64.5% (after 15 minutes treatment) or 26.3% (after 45 minutes treatment), respectively, thus ensuring the validity of the test system.
Compared with the result of the negative control, the relative absorbance values of the test item, corresponding to the cell viability, decreased after exposure to the cells. After the treatment period of 30 minutes, the cell viability was reduced to 22.0%, after 60 minutes only 16.1% of the cells survived. The 3-minutes exposure did not have any impact on the cell viability (103.7%).The calculated ET50-value was 20.7 minutes,consequently the test item was classified as moderate irritant.
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