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REACH

Benzyl phenylacetate

EC number: 203-008-4 | CAS number: 102-16-9

Life Cycle description
Uses advised against

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Benzyl phenylacetate
- Molecular formula (if other than submission substance): C15H14O2
- Molecular weight (if other than submission substance): 226.2736 g/mol
- Smiles notation (if other than submission substance):c1(CC(OCc2ccccc2)=O)ccccc1
- InChI:1S/C15H14O2/c16-15(11-13-7-3-1-4-8-13)17-12-14-9-5-2-6-10-14/h1-10H,11-12H2
- Substance type: Organic
- Physical state: Liquid

Analytical monitoring:

not specified

Details on sampling:

Sampling method:
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):dimethyl sulfoxide (DMSO).

Test organisms (species):

other: Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC))

Details on inoculum:

Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) from the Department of Dermatology, University of Pennsylvania.

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

24 h

Details on test conditions:

TEST SYSTEM
Test vessel: Agar plates
No. of organisms per vessel: microbial concentration of 106CFU/ml

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

other: MIC

Effect conc.:

> 2 000 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: effects conc. at inoculum 105CFU/plate for Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) species

Validity criteria fulfilled:

not specified

Conclusions:

The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) micro organisms species was found to be >2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to benzyl phenylacetate.

Executive summary:

The Minimum Inhibition (MIC) effect of test chemical Benzyl phenylacetate was observed on Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs. Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 10⁶CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

Description of key information

Key value for chemical safety assessment

Additional information

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