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EC number: 251-974-0 | CAS number: 34375-28-5
Endpoint summary
Administrative data
Description of key information
Biodegradation in water
Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance 2 -[hydroxy(methyl)amino]ethanol (CAS no. 34375-28-5) (EnviChem, 2014). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 68% degradation by BOD parameter in 14 days. Thus, based on percentage degradation, test substance 2 -[hydroxy(methyl)amino]ethanol was considered to be readily biodegradable in nature.
Biodegradation in water and sediment
Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound
2-[hydroxy(methyl)amino]ethanol (CAS No. 34375 -28 -5). If released in to the environment, 33.9% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of 2-[hydroxy(methyl)amino]ethanol in water is estimated to be 8.66 days (208 hrs). The half-life (8.66 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of 2 -[hydroxy(methyl)amino]ethanol in sediment is estimated to be 77.916 days (1870 hrs). Based on this half-life value, it indicates that 2 -[hydroxy (methyl)amino]ethanol is not persistent in sediment.
Biodegradation in soil
The half-life period of 2-[hydroxy(methyl)amino]ethanol (CAS No. 34375 -28 -5) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 66.1% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of 2 -[hydroxy(methyl)amino]ethanol in soil is estimated to be 17.33 days (416 hrs). Based on this half-life value of 2-[hydroxy (methyl) amino]ethanol, it is concluded that the chemical is not persistentin the soil environment and the exposure risk to soil dwelling animals is moderate to low.
Additional information
Biodegradation in water
Various experimental and predicted data for the target compound 2 -[hydroxy(methyl)amino]ethanol (CAS No. 34375 -28 -5) and various supporting weight of evidence studies for its read across substances were reviewed for the biodegradation end point which are summarized as below:
In an experimental study from secondary source (EnviChem, 2014), biodegradation experiment was conducted for 14 days for evaluating the percentage biodegradability of test substance 2 -[hydroxy(methyl)amino]ethanol (CAS no. 34375-28-5). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 68% degradation by BOD parameter in 14 days. Thus, based on percentage degradation, test substance 2 -[hydroxy(methyl)amino]ethanol was considered to be readily biodegradable in nature.
In a prediction using the Estimation Programs Interface Suite (EPI suite, 2017), the biodegradation potential of the test compound 2-[hydroxy(methyl)amino]ethanol(CAS No. 34375-28-5) in the presence of mixed populations of environmental microorganisms was estimated.The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that 2 -[hydroxy(methyl) amino]ethanol is expected to be readily biodegradable.
In a supporting weight of evidence study from authoritative database and secondary source (J-CHECK and OECD SIDS) for the read across chemical 1,4 -Butanediol (CAS no. 110 -63 -4),biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of read across substance 1,4 -Butanediol. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of read across substance was determined to be 83, 94 and 100% by BOD, TOC removal and GC parameter in 14 days. Thus, based on percentage degradation,1,4-Butanediol is considered to be readily biodegradable in nature.
For the read chemical hexane (CAS no. 110-54-3) from authoritative database (HSDB, 2016),biodegradation experiment was conducted for evaluating the percentage biodegradability of read across substance hexane (CAS no. 110-54-3) under aerobic conditions. Activated sludge was used as a test inoculum for the study. The percentage degradation of read across substance was determined to be 100% in less than 30 days. Thus, based on percentage degradation, hexane is considered to be readily biodegradable in nature.
Another biodegradation study was conducted for evaluating the percentage biodegradability of read across substance heptane (CAS no. 142-82-5) under aerobic conditions (from peer reviewed journal F. Solano-Serena et. al, 1999 and authoritative database HSDB, 2016). Activated sludge was used as a test inoculum obtained from urban waste water treatment plant. A microbial suspension containing 3 g/l dry weight was obtained by centrifugation of the sludge at 15000 g for 20 mins and resuspension of the centrifuged biomass in the same volume of nutrient solution. Inoculum conc. of 100 mg/l was used. The centrifuged biomass could be used immediately or stored at -80°C for several months without significant loss of degradation capacity. The vitamin – supplemented mineral salt medium was used as a nutrient solution. Individual hydrocarbons were added to the nutrient solution as sole carbon source. 500 ml flasks closed with Teflon-coated stoppers and with side-arms equipped with Mininert valves was used as a culture apparatus for the study. Test substance was added to 50 ml of inoculated culture medium through a valve with a Hamilton syringe. After an incubation period of 25 days at 30°C with an alternative shaking (70 strokes /min), 5 ml of CH2Cl2, containing 600 mg/ml dodecane as internal standard, was introduced into the flask through the valve and the remaining hydrocarbon were extracted for 1 h under shaking. The flasks were refrigerated overnight at 4°C before opening and the suspensions were centrifuged at 35000 g for 30 min at 4°C. The CH2Cl2 phase of each flask was then analyzed by gas chromatography. Abiotic controls containing 1 g/l HgCl2 were run under the similar test conditions. Carbon dioxide was measured with a chromatograph equipped with a thermal conductivity detector and a Porapak Q column (80/100 mesh, 2 m) using an external standard method. The carried gas was helium and the column temperature was 50°C. The temperature of the injector and that of the detector was 100°C. Samples (250µl) of the headspace gas of culture flasks were withdrawn with a gas-tight syringe and injected into the chromatograph for CO2 determination. The kinetics of O2 consumption during gasoline degradation were determined in duplicate at 30°C over 25 d with the electrolytic respirometric equipment D-12 Sapromat. The percentage degradation of read across substance was determined to be 17 and 100% by O2 consumption, CO2 production and GC parameter after 2 and 25 days, respectively. Thus, based on percentage degradation, heptane is considered to be readily biodegradable in nature.
On the basis of above results for target chemical 2-[hydroxy(methyl)amino]ethanol (from EnviChem, 2014 and EPI suite, 2017) and for its read across substance (from authoritative database J-CHECK, HSDB, peer reviewed journal and secondary source OECD SIDS), it can be concluded that the test substance 2 -[hydroxy(methyl)amino]ethanol can be expected to be readily biodegradable in nature.
Biodegradation in water and sediment
Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound
2-[hydroxy(methyl)amino]ethanol (CAS No. 34375 -28 -5). If released in to the environment, 33.9% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of 2-[hydroxy(methyl)amino]ethanol in water is estimated to be 8.66 days (208 hrs). The half-life (8.66 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of 2 -[hydroxy(methyl)amino]ethanol in sediment is estimated to be 77.916 days (1870 hrs). Based on this half-life value, it indicates that 2 -[hydroxy (methyl)amino]ethanol is not persistent in sediment.
Biodegradation in soil
The half-life period of 2-[hydroxy(methyl)amino]ethanol (CAS No. 34375 -28 -5) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 66.1% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of 2 -[hydroxy(methyl)amino]ethanol in soil is estimated to be 17.33 days (416 hrs). Based on this half-life value of 2-[hydroxy (methyl) amino]ethanol, it is concluded that the chemical is not persistentin the soil environment and the exposure risk to soil dwelling animals is moderate to low.
On the basis of available information, the test substance 2 -[hydroxy(methyl)amino]ethanol can be considered to be readily biodegradable in nature.
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