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EC number: 256-851-5 | CAS number: 50925-42-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- 1986
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weighings were used to prepare the different test item concentrations.
- Controls: For the reference compound a stock solution at a concentration of 500 mg/L
was prepared by dissolving 500 mg 3.5-Dichlorophenol in 10 ml of 1 M NaOH
and diluting to 1 litre with deionised water. The pH was adjusted to pH 7±0.5
with H2S04 - Test organisms (species):
- activated sludge of a predominantly industrial sewage
- Details on inoculum:
- - Laboratory culture: aboratory scale sewage treatment unit (3L), treating predominantly industrial sewage.
- Name and location of sewage treatment plant where inoculum was collected: Leverkusen Bürrig I Germany
- Preparation of inoculum for exposure:
- first, the sludge was settled and the supernatant was decanted, - after centrifuging (20 min at 4000 rpm and 20°C) the supernatant was decanted, - approx. 1 g of the wet sludge was dried in order to calculate the amount of sludge to achieve a concentration of activated sludge of 6 g/1 (dry weight) suspended solids, - the calculated amount of sludge was first dissolved in synthetic medium and than filled up to a defined end volume with deionised water, - Storage of sludge: aeration of the activated sludge at 20°c ± 2°C, daily fed with synthetic medium
- Initial biomass concentration: 6 g/L dry weight - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- no data
- Test temperature:
- 20.4 - 20.9
- pH:
- 7.1 - 7.9
- Dissolved oxygen:
- 10000 mg/L test concentration: 5.3 (mg 02/L) at start , 2.3 (mg 02/L) at end
- Salinity:
- no data
- Conductivity:
- no data
- Nominal and measured concentrations:
- 100, 1000 and 10000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 ml glass Erlenmeyer flasks
- Aeration: 3 hours with permanent aeration
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2 (1 at start and 1 at end)
- No. of vessels per oxygen consumption (replicates):1
- Sludge concentration (weight of dry solids per volume): 6 g/L dry weight
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic waste water feed was made by
dissolving the following amounts of substance in 1 litre of water.
16.0 g peptone
11.0 g meat extract
3.0 g urea
0.7 g NaCI
0.4 g CaCI2 x 2H20
0.2 g MgS04 X 7H20
2.8 g K2HP04
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Oxygen consumption, temperature and ph were measured after an aeration time of 3 hours - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- Concentration: 1 and 20 mg/L
Inhibition: 0 at 1 mg/L, 69.2 at 20 mg/L - Validity criteria fulfilled:
- yes
- Remarks:
- (respiratory rates of the 2 controls differ less than 15%, ECso of the reference compound 3,5-Dichlorophenol is in the range 5- 30 mg/L)
- Conclusions:
- Bayscript Gelb BR showed 1. 7% respiration inhibition of activated sludge at the highest test item concentration of 10000 mg/L. The ECso:was > 10000 mg/L.
- Executive summary:
To assess the toxicity of Bayscript Gelb BR to bacteria a study was conducted in accordance with ISO 8192-1986/B "Activated sludge respiration inhibition" (1986). This test method is equal to OECD Guideline 209 (1984). The activated sludge was exposed to Bayscript Gelb BR at different concentrations (100, 1000, 10000 mg/L). The respiration rate of each mixture was determined after aeration periods of 3 hours.
Before use the pH of the activated sludge was measured and adjusted to pH 6-8. 8 ml of the synthetic medium and 25 ml of activated sludge were added to the dissolved test item. The mixture was filled up with deionised water to 250 ml and aerated at 20°C ± 2°C. The exposure medium with the reference substance was prepared by adding 8 ml of the synthetic medium, 25 ml of activated sludge and a defined amount of the stock solution to achieve the test concentrations and was filled up to 250 ml with
deionised water. Oxygen consumption was measured and recorded after an aeration time of 3 hours. Thereafter, temperature and pH of the exposure medium were measured. A control at test start and end (control 1, 2) was tested in the same way. The exposure medium to determine the physico-chemical oxygen consumption was prepared as the medium with the test item, but without activated sludge.
Bayscript Gelb BR showed 1.7% respiration inhibition of activated sludge at a test item concentration of 1 0000 mg/L. The ECso is > 10000 mg/L. The effect value related to nominal concentration, since no analytical monitoring was performed.
Reference
Description of key information
Bayscript Gelb BR showed 1. 7% respiration inhibition of activated sludge at the highest test item concentration of 10000 mg/L. The EC50 was > 10000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
should read > 10000 mg/L
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