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EC number: 923-725-2
CAS number: -
Biofert Plusz showed a low toxicity to Eisenia fetida in a study conducted according to the guidelines OECD 222, ISO 11268-2, and BBA (28 d NOEC (mortality, body weight)=10 g test material/kg soil dry weight; 28 d LOEC (mortality, body weight)=20 g test material/kg soil dry weight; 56 d NOEC (reproduction)=2.5 g test material/kg soil dry weight; 56 d LOEC (reproduction)=10 g test material/kg soil dry weight; 56 d EC50 (reproduction)=3.23 g test material/kg soil dry weight).
In a study conducted according to OECD Guideline 222 (Earthworm
Reproduction Test (Eisenia fetida/Eisenia andrei)), ISO 11268-2 (Effects
of Pollutants on Earthworms. 2. Determination of Effects on
Reproduction), and BBA Guideline (Guidelines for the testing of
herbicides within registration progress - Effects of herbicides on
reproduction and growt of Eisenia fetida/Eisenia andrei; 1994), the
influence of Biofert Plusz on mortality, biomass, reproduction,
behaviour and pathological symptoms of earthworms was investigated.
Forty earthworms (4 replicates: 10 adults each), Eisenia fetida, were
exposed to Biofert concentrations ranging from 1.25 to 20 g test
material/kg soil dw for 28 d under static conditions.Thereafter, the
adults were removed from the test vessels and mortality and body weight
determined. After further 4 weeks the reproduction rate was determined
by counting the hatched juvenils. The 28 d NOEC and LOEC based on
mortality and body weight were determined to be 10 and 20 g test
material/kg soil dry weight, respectively. The 56 d NOEC and LOEC based
on reproduction were determined to be 2.5 and 5 g test material/kg soil
dry weight, respectively. Furthermore, a 56 d EC50 (reproduction)=3.23 g
test material/kg soil dry weight was found. Furthermore, no
biological significant symptoms or changes in the behaviour of the
earthworms were observed up to a test concentration of 10 g test
material/kg soil dw after 28 d.
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