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EC number: 239-405-4 | CAS number: 15373-31-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-05-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to OECD Guideline and GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Bovine Corneal Opacity and Permeability (BCOP) Assay, SOP of Microbiological Associates Ltd., UK, Procedure Details, April 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,2,3-trimethylcyclopent-3-enylacetonitrile
- EC Number:
- 239-405-4
- EC Name:
- 2,2,3-trimethylcyclopent-3-enylacetonitrile
- Cas Number:
- 15373-31-6
- Molecular formula:
- C10H15N
- IUPAC Name:
- 2-(2,2,3-trimethylcyclopent-3-en-1-yl)acetonitrile
Constituent 1
Test animals / tissue source
- Species:
- other: bovine
- Details on test animals or tissues and environmental conditions:
- - Freshly isolated bovine cornea (at least 9 month old donor cattle)
- Source: Schlachthof Bensheim, 64625 Bensheim, Germany
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied: 0.75 mL
- Concentration: undiluted - Duration of treatment / exposure:
- 10 min
- Observation period (in vivo):
- not applicable
- Number of animals or in vitro replicates:
- 3 cornea per substance
Results and discussion
In vivo
Results
- Irritation parameter:
- other: IVIS
- Basis:
- mean
- Time point:
- other: 10 min
- Score:
- 0.23
- Reversibility:
- other: not applicable
- Remarks on result:
- other: BCOP test result
Any other information on results incl. tables
Results after 10 min incubation time
Test group |
Opacity value = Difference (t130-t0) of Opacity |
Permeability at 490 nm (OD490) |
IVIS |
Mean IVIS |
Proposed in vitro Irritancy Score |
||
|
|
Mean |
|
Mean |
|
|
|
Negative control |
-1 |
-0.33 |
0.082 |
0.068 |
0.23 |
0.69 |
Not categorized |
0 |
0.065 |
0.98 |
|||||
0 |
0.058 |
0.87 |
|||||
Positive control |
55.33 |
1.348 |
70.55 |
79.37 |
Category 1 |
||
67.33 |
1.395 |
88.25 |
|||||
53.33 |
1.731 |
79.25 |
|||||
Test item |
0.33 |
0 |
0.33 |
0.23 |
Not categorized |
||
0.33 |
-0.006 |
0.24 |
|||||
0.33 |
-0.014 |
0.12 |
Opacity and permeability values of positive control and the test substance are shown as corrected values.
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- Migrated information
- Conclusions:
- From the results of the in vitro BCOP study it was concluded that the test substance is not to be categorized.
- Executive summary:
An in vitro study with fresh bovine cornea was conducted according to OECD 437 to investigate the eye irritation properties of the test substance. After a first opacity measurement of the fresh bovine corneae (t0), the neat test item, the positive and the negative controls were applied to corneae and incubated for 10 minutes at 32 ± 1 °C. After the incubation phase the substances were rinsed from the corneae. The corneae were incubated for another 120 minutes at 32 ± 1 °C in incubation medium, and opacity was measured a second time (t130). After the opacity measurements permeability of the corneae was determined by measuring spectrophotometrically the transfer of sodium fluorescein after incubation in a horizontal position for 90 minutes at 32 ± 1 °C. With the negative control (saline) neither an increase of opacity nor permeability of the corneae could be observed (mean IVIS = 0.69). The positive control (2-Ethoxyethanol) was tested undiluted and showed clear opacity and distinctive permeability of the corneae (mean IVIS = 79.37) corresponding to a classification as serious eye damaging (CLP Cat 1). The test item was tested undiluted. Relative to the negative control, the test item did not cause an increase of the corneal opacity or permeability. The calculated mean IVIS was 0.23 (threshold for serious eye damage: IVIS≥55). According to OECD 437 the test item is not categorized.
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