REAKTIVE GELB F 97494

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From November 5, 2001 to November 9, 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: The analytical concentration of the test substance was determined at the following time points - 0, 24, 48, 72 and 96 h.

Vehicle:

no

Details on test solutions:

FINAL TEST SOLUTION:
- Test solution: 500 mg of the test substance was added to 5 L of dilution water to get a nominal concentration of 100 mg/L.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: Hamilton-Buchanan
- Source: Bio International B. V. (Netherlands)
- Age at study initiation (mean and range, SD): 2 months
- Length at study initiation (length definition, mean, range and SD): 2.9 cm (SD = 0.24 cm)
- Feeding during test: No feeding during exposure period
ACCLIMATION
- Acclimation period: 5 months
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Commercial fish food
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): mortality ≤2%

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

246.3 mg CaCO3/L

Test temperature:

20-24°C

pH:

Control: 7.9 to 8.1
Test: 7.9 to 8.0

Dissolved oxygen:

>5 mg/L

Salinity:

13.8°dH (=246.3 mg/L CaCO3)

Nominal and measured concentrations:

Nominal concentration: 100 mg/L
Measured concentration: Measured concentrations correspond to 105.9% of nominal values at 0 h, 101.7% of nominal values at 24 h, 105.9% of nominal values at 48 h, 105.9% of nominal values at 72 h, and 110.1% of nominal values at 96 h respectively.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria holding 5 L of test media
- Aeration: gentle aeration via narrow glass tubes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.85 g body weight (wet weight) per litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water prepared according to the recommendations of ISO 7346. The total hardness of the dilution water, measured at test start, was 13.8 °dH (=246.3 mg/L CaCO3)
- Total organic carbon: <2 mg/L

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 105.9 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 105.9 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality in control: 0/10

Sublethal observations / clinical signs:

Results:

 

Time [h]	LC 0 [mg/L] (test substance)
2	≥105.9
24	≥103.8
48	≥104.5
72	≥104.8
96	≥105.9

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 96 h LC50 and NOEC of the test substance were found to be >100 mg/L (nominal concentration).

Executive summary:

A study was conducted to determine the acute toxicity of the test substance to Zebra fish (Brachydanio rerio) according to EU Method C.1 (which is in most parts equivalent to the OECD Guideline 203), in compliance with GLP.

 

Groups of ten fish were exposed to a nominal concentration of 100 mg/L of the test substance dissolved in water. The concentration was determined at 2, 24, 48, 72 and 96 h. Measured concentrations correspond to 105.9% of nominal values at 0 h, 101.7% of nominal values at 24 h, 105.9% of nominal values at 48 h, 105.9% of nominal values at 72 h and 110.1% of nominal values at 96 h, respectively. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 h of exposure. No mortality was observed in the control or test substance treated groups. Under the test conditions, the 96 h LC50 and NOEC of the test substance were found to be ≥100 mg/L (nominal concentration).

Description of key information

Under the test conditions, the 96 h LC50 and NOEC of the test substance were found to be ≥100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L

Additional information

A study was conducted to determine the acute toxicity of the test substance to Zebra fish (Brachydanio rerio) according to EU Method C.1 (which is in most parts equivalent to the OECD Guideline 203), in compliance with GLP. Groups of ten fish were exposed to a nominal concentration of 100 mg/L of the test substance dissolved in water. The concentration was determined at2, 24, 48, 72 and 96 h.Measured concentrations correspond to 105.9% of nominal values at 0 h, 101.7% of nominal values at 24 h, 105.9% of nominal values at 48 h, 105.9% of nominal values at 72 h and 110.1% of nominal values at 96 h, respectively.Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 h of exposure.No mortality was observed in the control or test substance treated groups. Under the test conditions, the 96 h LC50 and NOEC of the test substance were found to be ≥100 mg/L.