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EC number: 440-550-2 | CAS number: 244021-01-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Performed according to OECD-Guideline under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Details on test material:
- Identity: TKA 40270 (CGPS 345)
Batch no.: Lot 02243FC7, Op. 1/07
Purity: Confidential Information (see Analytical Certificate No: 38291-1)
Expiration date: 01-Oct-2012
Solubility in water: <0.21 mg/L
Aggregate state / physical form at room temperature: Liquid
Color: Colorless to yellow
Storage conditions: Storage at under 18 °C may lead to partial solidification. To reliquify, heat contents to 25-35 °C.Protect from light. (At RCC: At room temperature at about 20 °C, away from direct sunlight.)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Nominal concentration test 100 mg/L (Limit Test)
- Sampling after 3 hours incubation time; then O2 was continuously measuared
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The final nominal concentration tested was 100 mg/L. No stock solution could be prepared due to the low water solubility of the test item.
The test item was handled in the dark due to its light sensitivity. Therefore, 50.07 mg of the test item was first weighed onto a glass slide and then
transferred to the designated test vessel. Then, 284 ml, tap water was added to each vessel.
The test item was mixed into the tap water by ultrasonic treatment for fifteen minutes and intense stirring for 24 hours at room temperature in the
dark to dissolve a maximum amount of the test item and/or disperse it as homogeneously as possible. No emulsifiers or solvents were used.
After stirring, the appearance of the test medium was evaluated, and the test concentration was clearly above the water solubility limit of TKA 40270
(CGPS 345) under the test conditions. Then, 16 mL synthetic wastewater and 200 mL activated sludge inoculum were added (see table on next page).
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- The study was performed with aerobic activated sludge from a wastewater treatment plant treating predominantly domestic wastewater. The sludge
was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
Based on this ratio, an aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material per liter. During
the holding period of three days prior to use, the sludge was fed daily with 50 mL synthetic wastewater per liter and was kept at room temperature
under continuous aeration until use, Before use, the dry weight of the activated sludge was measured again in the inoculum used for the test, The pH of the activated sludge inoculum was 7.4.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- at least 10 minutes
Test conditions
- Hardness:
- Synthetic wastewater was used
- Test temperature:
- 20°C
- pH:
- The pH was 7.5 - 8.1 for the test solution
- Dissolved oxygen:
- 8.7 mg/L
- Nominal and measured concentrations:
- Nominal: 100 mg/L
- Details on test conditions:
- At the start of the test (after the stirring period of 24 hours), synthetic wastewater and activated sludge inoculum were added. The inoculum had a
sludge concentration of 3.4 g/L dry weight (corresponding to about 1.4 g dry material per liter test medium). The sludge was added in time intervals of 15 minutes (an arbitrary but convenient interval) first to a control, secondly to the test solutions of the reference item, thirdly to the test suspension of the test item, and finally to the second control.
During the incubation period of 3 hours the single test medium and the controls were continuously aerated by intense stirring on magnetic stirrers
to avoid possible foaming and/or stripping of the test item. The concentration of dissolved oxygen did not drop below 2.5 mg/L during the
incubation period. Just before measurement of the respiration rates the dissolved oxygen concentrations were at least 8.7 mg/L. The temperature in the test media, measured in one control, was 20 °C at the start and at the end of the incubation period. Then the dissolved oxygen concentration was measured with an oxygen electrode, and was continuously recorded. During measurement, the samples were continuously stirred on a magnetie
stirrer. The oxygen consumption rate (in mg 02 /L minute) was determined from the linear part of the respiration curve in the range 6.5 - 2.5 mg
02/L. In case of very rapid oxygen consumption, the range used was below the limits indicated above but always within the linear part of the
respiration curve. In case of low oxygen consumption the rate was determined over a period of at least ten minutes. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The test item TKA 40270 (CGPS 345) had no significant inhibitory effect (=15%) on the respiration rate of activated sludge after the incubation period of 3 hours at the limit test concentration of 100 mg/L. Based on the physical appearance of the test suspension, the saturation concentration (water solubility limit of TKA 40270 (CGPS 345) under the test conditions) was <100 mg/L and resulted in less than 15% inhibition after three hours contact time.
Thus, the 3-hour NOEC (EC15) of TKA 40270 (CGPS 345) to activated sludge microorganisms was at least 100 mg/L. This value might even be higher but concentrations above 100 mg/L were not tested. The 3-hour EC20, EC50, and EC80 could not be calculated but were clearly higher than
100 mg/L. - Results with reference substance (positive control):
- The reference item 3,5-dichlorophenol was tested in parallel under identical test conditions, and functioned as a positive control. The nominal
concentrations tested were 5, 16 and 50 mg/L. A stock solution of 3,5-dichlorophenol was prepared according to the test guidelines:
0.5 g 3,5-dichlorophenol was dissolved in 10 mL 1 M NaOH and diluted to about 30 mL with purified water. Excess NaOH was neutralized with
0.5 mol/L H2SO4 to the point of incipient precipitation.
The 3-hour EC5O of the reference item 3,5-dichlorophenol was calculated to be 15 mg/L (the 95% confidence limits could not be calculated). The
3-hour EC5O is within the guideline-recommended range of 5—30 mg/L, confirming suitability of the activated sludge used.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-hour NOEC (EC15) of the test item to activated sludge microorganisms was at least 100 mg/L. This value might even be higher but concentrations above 100 mg/L were not tested. The 3-hour EC20, EC50, and EC80 could not be calculated but were clearly higher than 100 mg/L.
- Executive summary:
A limit test was performed in accordance with the EU Commission Directive 92/69/EEC (C.1-C.3, [1]) to demonstrate that the test item has no toxic effect on activated sludge up to the nominal test concentration of 100 mg/L. The respiration rates (oxygen consumption) of activated sludge were measured. The respiration rate in the presence of the test item was compared to the mean respiration rate of two controls.
At the test item concentration of 100 mg/L, the test item was not fully dissolved in the test medium. Thus, the test concentration was clearly above the water solubility limit of TKA 40270 (CGPS 345) under the test conditions. The test item TKA 40270 (CGPS 345) had no significant inhibitory effect (=15%) on the respiration rate of activated sludge after the incubation period of 3 hours at the limit test concentration of 100 mg/L. Based on the physical appearance of the test suspension, the saturation concentration (water solubility limit of TKA 40270 (CGPS 345) under the test conditions) was reached and no inhibitory effect was determined after three hours contact time.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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