Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
The main structure of the read-across substance is the same of the CJ304, only the salt form is different. However, this difference does not influence the toxicity of the CJ304. CJ304 contains Li/Na salt form and the read-across substances contains only Na salt. This will not lead to difference on the toxicity results.

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
Version / remarks:
Method B-21
Deviations:
no
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Specific details on test material used for the study:
Name: FAT 41001/F
Purity: >90%

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
activated sludge

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

pH:
7.4 - 7.6
Dissolved oxygen:
4.7 - 5.1
Nominal and measured concentrations:
1, 3.2, 10, 32, 100 and 320 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorphenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Results with reference substance (positive control):
3h IC50 for 3,5 - Dichlorophenol = 11 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The IC50 of FAT 41001/F was found to be greater than 320 mg/L.
Executive summary:

A study was performed with FAT 41001/F to determine the toxicity to microorganisms. This test was carried out in accordance to OECD test guideline 209 and CIBA internal method B-21.

The concentration of test substance used: 1, 3.2, 10, 32, 100, 320 mg/L. 3,5 - Dichlorophenol was used as reference substance.

Based on the findings of the study, the 3h IC50 for FAT 21030/A was found to be greater than 320 mg/L. The 3h IC50 of reference substance was determined to be 11 mg/L.