Ethyl benzoate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl benzoate

EC Number:

202-259-7

EC Name:

Methyl benzoate

Cas Number:

93-58-3

Molecular formula:

C8H8O2

IUPAC Name:

methyl benzoate

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Administration / exposure

Type of coverage:

not specified

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST MATERIAL
- Amount applied: 2000 mg/kg bw

Duration of exposure:

single exposure, test substance was not washed off afterwards

Doses:

one dose, 2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were weighted on days 1, 2, 4, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, histopathology

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality occurred.

Clinical signs:

other: other: No treatment-related findings were recorded.

Gross pathology:

No abnormal findings were recorded.

Other findings:

- Other observations: Cysts in the oviduct were found in three females which were considered to be normal for this strain of rabbits and therefore not treatment-related.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Methyl bezoate was tested for its dermal toxicity in 10 New Zealand White rabbits. It was shown that a single dose of 2000 mg/kg bw did not lead to any deaths, and therefore the LD 50 was found to be >2000 mg/kg bw.

Executive summary:

Methyl bezoate was tested for its dermal toxicity in 10 New Zealand White rabbits (5 males and 5 females). Therefore, a single dose of 2000 mg/kg bw was applied to the rabbits' skin. Loss of test substance was prevented by using a collar. The animals were weighted (on days 1, 2, 4, 7 and 14) and observed during the 14 -day observation period. No deaths occurred. The treated skin sites showed signs of irritaion. A decrease in body weights was observed in four male and three female rabbits on day 1 and one female showed a decrease in body weight during days 4 and 7. At necropsy, no treatment related findings were observed. Therefore, the LD50 after dermal application was found to be greater than 2000 mg/kg bw.