N-(trans-4-Hydroxycyclohexyl)-3,5-dibromo-2-hydroxybenzylidenamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17.06.2003-15.07.2003

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dibromo-6-({[(1r,4r*)-4-hydroxycyclohexyl]imino}methyl)phenol

EC Number:

924-083-6

Molecular formula:

C13 H15 Br2 N O2

IUPAC Name:

2,4-dibromo-6-({[(1r,4r*)-4-hydroxycyclohexyl]imino}methyl)phenol

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Results and discussion

% Degradation

Parameter:

other: biochemical oxygen demand (BOD)

Value:

>= 43 - <= 49

Sampling time:

28 d

Applicant's summary and conclusion

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

Consequently, NA 1523 was found to be moderately biodegradable (43 - 49%) under the
test conditions within 28 days. However, the pass Ievei for ready biodegradability, i.e. a
biodegradation of at least 60% of the ThOD in a 1 0-day window within the 28-day period of
the testwas not reached.

Executive summary:

The test item NA 1523 was investigated for its ready biodegradability in a manometric respirometry test over 28 days based on EU Commission Directive 92/69 EEC, C.4-D (1992) and OECD Guideline for Testing of Chemieals No. 301 F (1992). The percent biodegradation of the test item was calculated based on the theoretical oxygen demand of 1.23 mg 02/mg test item without nitrification (Th0DNH4) and 1.40 mg 0 2/mg test item with nitrification (ThODNo3). The biochemical oxygen demand (800) of NA 1523 in the test media slowly but steadily increased from about Day 8 until test termination after 28 days. At the end of the 28-day exposure period, the mean biodegradation of NA 1523 amounted to 49% without nitrification and 43% with nitrification. Consequently, NA 1523 was found to be moderately biodegradable (43 - 49%) under the test conditions within 28 days. However, the pass Ievei for ready biodegradability, i.e. a biodegradation of at least 60% of the ThOD in a 1 0-day window within the 28-day period of the testwas not reached. ln the toxicity control, containing both NA 1523 and the reference item sodium benzoate, no inhibitory effect on the biodegradation of the reference item was determined. Thus, NA 1523 had no inhibitory effect on the activity of activated sludge microorganisms. ln the procedure controls, the reference item sodium benzoate was biodegraded by an average of 85% on exposure Day 14, and reached an average biodegradation of 87% by the end of the test (Day 28), thus confirming suitability of the activated sludge.