Registration Dossier
Registration Dossier
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EC number: 200-755-8 | CAS number: 71-48-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Publication which meets basic scientific principles with acceptable restrictions (e.g. no purity of the test substance are given).
Data source
Reference
- Reference Type:
- publication
- Title:
- Grenzwerte der Schadwirkung wassergefährdender Stoffe gegen Bakterien (Pseudomonas putida) und Grünalgen (Scenedesmus quadricauda) im Zellvermehrungshemmtest.
- Author:
- Bringmann G, Kühn R
- Year:
- 1 977
- Bibliographic source:
- Z Wasser Abwasser Forsch 3: 87-98
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Alga growth inhibition test according to Bringmann & Kühn
- GLP compliance:
- no
Test material
- Reference substance name:
- Acetic acid
- EC Number:
- 200-580-7
- EC Name:
- Acetic acid
- Cas Number:
- 64-19-7
- IUPAC Name:
- acetic acid
- Details on test material:
- - No further details on the test substance characteristics are given.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- Scenedesmus quadricauda
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 8 d
Test conditions
- Test temperature:
- 27 °C
- pH:
- neutralized
- Details on test conditions:
- CULTURE CONDITIONS
- Culture vessels: 100 ml Erlenmeyer flasks
- Culture volume: 20 ml (nutrient solution 1)
- Light conditions: continous white light
- Temperature: 27 °C
- Pre-cultures: in sterilised Erlenmeyer flasks in 20 ml medium
TEST CONDITIONS
- Dilution water chemistry: sterile bidest. water
- Exposure vessels: 18 x 180 mm Kapsenberg culture vessels
- Exposure volume: 10 ml
- Number of replicates: 3
- Initial cell number: E(575 nm) = 20 for 10 mm layer thickness
- Exposure duration: 8 days
- Test temperature during the test: 27 °C
- pH values during the test: neutralised
- Photometric determination of absorbance at 578 nm after 8 days.
Results and discussion
Effect concentrations
- Duration:
- 8 d
- Dose descriptor:
- other: TTC
- Effect conc.:
- 4 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: neutralized, TTC (toxic threshold concentration) which is comparable with EC5
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
