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EC number: 942-520-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish, juvenile growth test
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June - September 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- This study was performed for the notification in other regions where authorities do not accept read-across data or QSAR.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 215 (Fish, Juvenile Growth Test)
- Version / remarks:
- January 21, 2000
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling method: once a week, fresh and old media
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.00 g test item were weighed and added into a glass bottle filled with 10 l test water. The bottle was capped and the solution was stirred slowly by magnetic stirrer at a rate of 200-230 rpm for 48 +/- 1 hours and allowed to stand for 50-70 minutes. After der settling period, about 5 l of the solution was drawn off from the depth of about 7 cm above the bottom of the bottle and then used as test solution for exposure.
- Controls: concurrent water without test substance - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: own bred in the testing facility
- Weight at study initiation (mean and range, SD): 0.050-0.10 g
- Food type: brine shrimp nauplii, newly hatched
- Amount: 4% of body weight
- Frequency: once daily
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Hardness:
- 190 mg/l (as CaCO3)
- Test temperature:
- 21.1 - 23.1 °C
- pH:
- 7.70 - 8.24
- Dissolved oxygen:
- 75.9 - 99.8 %
- Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass bottles
- Size of vessel: diameter: 16 cm, height: 26 cm
- Renewal rate of test solution (frequency): daily
- No. of organisms per vessel: 10
- Loading rate: 0.2 -1.0 g fish/L
- Volume of test solution: 5 L
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Test temperature: 21.1 - 23.1 °C
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/l
- Test conditions: renewal of test solution every 2 or 3 days
- Results used to determine the conditions for the definitive study: No deaths and visible toxic signs were observed in the control and test groups. No specific differences between control and test group on pseudo-specific growth rate (fish weights of initial day and day 28) - Reference substance (positive control):
- no
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- weight
- Details on results:
- - Observed effects: see Table 1
- Cumulative mortality at each concentration and for each recommended observation time if possible: no deaths
- Mortality in the controls: no deaths
- Toxic signs: none in control and test groups
- Weights: The individual weights were in the range of 0.0752-0.0956g at the start. For the whole batch of fish used in the test, the range of individual weights at the start of the test was -12.0 % - + 11.8 % of the arithmetic mean weight (0.0855 g). After exposure for 28 d, the mean weight of fish in the control has increased by 136.6 %of their mean initial weight over 28 days, which met the validity criterion of the test. The mean weight of fish in the test group has increased by 124.8 % of their mean initial weight over 28 days. Compared with the average r3 for the control, the percent inhibition of the average r3 for the test group was 5.9%. The result of the two-sided Student t-test indicated that r3 did not display significant differences (p>0.05) between the control and the test group. - Reported statistics and error estimates:
- Two-sided Student t-test with a 5% significance level was used to compare the pseudo-specific growth rate of the test group with the control and to obtain the lowest observed effect loading rate (LOEL) and the no observed effect loading rate (NOEL).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the growth of juvenile zebra fish, the 28-day NOEL of the test substance Guerbet alcohols, 24-26, branched and cyclic was determined to be >= 100 mg/l and the 28-day LOEL was > 100 mg/l.
- Executive summary:
A fish, juvenile growth test of Guerbet alcohols, 24-26, branched and cyclic was conducted with zebra fish (Danio rerio) over a 28-d exposure period at a loading rate of 100 mg/l. The test was performed under semi-static conditions and test solutions were renewed daily. During the 28-day exposure period, no deaths and visible toxic signs were observed in the control and the test group. At the start and the end, the fish were weighd individually as wet weights (blotted dry). The pseudo-specific growth rate "r3" was calculated to evaluate toxic effetcs. Two-sided Student t-test with a 5% significance level was used to compare "r3" of the test group with the control and to obtain the lowest observed effect loading rate (LOEL) and the no observed effect loading rate (NOEL). The result of the two-sided Student t-test indicated that r3 did not display significant differences (p> 0 .05) between the control and the test group.
Reference
Table 1: Pseudo-specific growth rate r3 after 28 days in the main test
Loading rate | Control | 100 mg/l |
Fish 1 r3 (per day) |
3.15 % |
3.16 % |
Fish 2 r3 (per day) |
2.93 % |
2.95 % |
Fish 3 r3 (per day) |
2.72 % |
2.98 % |
Fish 4 r3 (per day) |
3.56 % |
2.55 % |
Fish 5 r3 (per day) |
3.15 % |
3.37 % |
Fish 6 r3 (per day) |
3.47 % |
2.63 % |
Fish 7 r3 (per day) |
3.22 % |
2.57 % |
Fish 8 r3 (per day) |
2.88 % |
2.62 % |
Fish 9 r3 (per day) |
2.83 % |
2.66 % |
Fish 10 r3 (per day) |
2.73 % |
3.31 % |
Arithmetic mean r3 |
3.06 % |
2.88 % |
Inhibition of r3 (%) |
- |
5.9 % |
p-value (t-test) |
- |
0.20 |
Description of key information
A fish, juvenile growth test of Guerbet alcohols, 24-26, branched and cyclic was conducted with zebra fish (Danio rerio) over a 28-d exposure period at a concentration of 100 mg/l. The test was performed under semi-static conditions and test solutions were renewed daily. During the 28-day exposure period, no deaths and visible toxic signs were observed in the control and the test group. At the start and the end, the fish were weighd individually as wet weights (blotted dry). The pseudo-specific growth rate "r3" was calculated to evaluate toxic effetcs. Two-sided Student t-test with a 5% significance level was used to compare "r3" of the test group with the control and to obtain the lowest observed effect loading rate (LOEL) and the no observed effect loading rate (NOEL). The result of the two-sided Student t-test indicated that r3 did not display significant differences (p> 0 .05) between the control and the test group.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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