Registration Dossier
Registration Dossier
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EC number: 200-070-4 | CAS number: 50-89-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
A study was carried out according to EU Method C.1, OECD Guideline 203 (Fish, Acute Toxicity Test) and OPPTS 850.1075. The purpose of this study was to evaluate the acute toxicity of the test item on Zebrafish (Brachydanio rerio). Young fish were exposed to aqueous test media containing the test item for 96 hours. Based on results obtained in a preliminary experiment fish were exposed to a single concentration of 100 mg/L (limit test). A concurrent control group was run.
A static test was performed as the test item was previously shown to be stable in the test medium over the test period. The analytically measured test item concentration remained within ± 20 % of the nominal during the test period of 96 hours. Therefore, biological results are based on nominal concentrations.
The fish were observed at approximately 3, 6, 24, 48, 72 and 96 hours after start of the test for signs of intoxication and mortality. Measurements of pH, dissolved oxygen and temperature were carried out daily. The test medium was fully characterized.
All validity criteria were met. Mortality and any sub-lethal effects were not observed during the 96-h exposure period either in the treated or in the control group. The 96-h NOEC and LC0 values were determined to be 100 mg/L; the LC50 the LOEC, and LC100 values were determined to be > 100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
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