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EC number: 619-057-3 | CAS number: 94667-33-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24/04/2001- 30/07/2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No information provided. - Analytical monitoring:
- yes
- Details on sampling:
- Water samples were collected from each test chamber at test initiation, at appoximately 48 hours and at test termination to measure concentrations of the test substance. Samples were collected from mid-depth of the test chambers. All samples were analyzed as soon as possible without storage.
Temperature, dissolved oxygen and pH were also measured in each test chamber daily during the test.
- Vehicle:
- not specified
- Details on test solutions:
- A 1.6 mg a.s./l primary stock solution was prepared by dissolving the test substance in reverse osmosis water. The primary stock solution was further diluted with reverse osmosis water to prepare four additional secondary stock solutions at concentrations of 8.0, 4.0, 2.0 and 1.0 mg a.s./l.
The working stock solutions were injected into the diluter mixing chambers at a rate of 0.0125 ml/minute, where they were mixed with dilution water at a rate of 125 ml/minute to achieve the desired test concentrations. The diluter was adjusted so that each test chamber received approximately 6 volume additions of test water every 24 hours. - Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- Test organism: Bluegill (Lepomis macrochirus)
Source of test organisms: Osage Catfisheries Inc.
Age of test organisms: Juvenile
Fish acclimated to test conditions for approximately 50 hours prior to test initiation
Temperature: 21.9oC- 22.5oC
pH: 7.9-8.2
D.O. 8.0- 8.2 mg/l - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- The test animals were observed for 96 hours following the start of exposure.
- Hardness:
- 128-132 mg/l CaCO3
- Test temperature:
- 22 ± 2°C
- pH:
- 7.9-8.2
- Dissolved oxygen:
- > 7.2 mg/l (*2% saturation)
- Salinity:
- No information provided.
- Nominal and measured concentrations:
- Nominal concentrations of the test substance: 0, 0.10, 0.20, 0.40, 0.80 and 1.6 mg a.s./l
Measured concentrations of the test substance: 0, 0.090, 0.19, 0.38, 0.77 and 1.4 mg a.s./l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 25l Teflon coated stainless steel aquaria
Effect parameter measured: Mortality.
Instantaneous loading: 0.72 g fish/l
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Test concentrations were doubled as they increased.
- Justification for using less concentrations than requested by guideline: No information provided.
- Test concentrations: Initial concentration of the test substance: 0, 0.10, 0.20, 0.40, 0.80 and 1.6 mg a.s./l
Actual concentrations of the test substance: 0, 0.090, 0.19, 0.38, 0.77 and 1.4 mg a.s./l
- Results used to determine the conditions for the definitive study: No information provided. - Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence limits: 0.38 mg/L, 0.77mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 0.77 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- The results of this study indicate that Bardap 26 can be toxic to Bluegill over a 96hr period. The no-observed-effect concentration (NOEC) was calculated to be 0.19 mg a.s./l. The 96hr LC100 was found to be 0.77 mg a.s./l. The 96hr LC50 was calculated to be 0.52 mg a.s./l with 95% confidence limits of 0.38 mg a.s./l and 0.77 mg a.s./l. No behavioural abnormalities or other adverse effects were observed during the study.
- Results with reference substance (positive control):
- No information provided.
- Reported statistics and error estimates:
- No information provided.The data were analyzed using the computer program of C.E. Stephan. The 24, 48 and 96 hour LC50 values were calculated by binomial probability with nonlinear interpolation. The 72 hour LC50 was calculated by probit analysis. The NOEC was determined by visual interpretation of the mortality and clinical observation data.
- Sublethal observations / clinical signs:
Table 1. Cumulative mortality
Nominal concentration (mg a.s./l)
Cumulative Mortality
% Mortality
5 hours
24 hours
48 hours
72 hours
96 hours
96 hours
0
0
0
0
0
0
0
0.090
0
0
0
0
0
0
0.19
0
0
0
0
0
0
0.38
0
0
0
1
1
5
0.77
5
18
19
19
10
100
1.4
120
20
20
20
20
100
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on concentration effect relationship observed, the no-observed-effect concentration (NOEC) was found to be 0.19 mg/l under the conditions of this study, the LC50 to be 0.52 mg a.s./l and the LC100 to be 0.77 mg a.s./l.
- Executive summary:
A 96-hour acute toxicity study was conducted to determine the ability of N,N-Didecyl-N-methylpoly(oxyethyl)ammonium Propionate (Bardap 26) to cause acute toxic effects to fish when tested in accordance with EPA OPPTS 850.1075. The test animals used were Bluegill (Lepomis macrochirus), exposed to the test material for 96 hours in a flow-through test system at concentrations of 0, 0.10, 0.20, 0.40, 0.80 and 1.6 mg a.s./l. (nominal concentrations: 0, 0.090, 0.19, 0.38, 0.77 and 1.4 mg a.s./l).
100% mortality was observed at the 0.77 and 1.4 mg a.s./L concentration groups and 5% mortality was recorded in the 0.38 mg a.s./L test group. No adverse effects were observed in any test group in the surviving fish. Based on concentration effect relationship observed, under the conditions of this study, the no-observed-effect concentration (NOEC) was found to be 0.19 mg/l.
Reference
Description of key information
A key study and a supporting study are provided for the assessment of short-term toxicity to fish. Both studies are conducted in accordance with GLP, with no deviations from the guidelines.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 0.52 mg/L
Additional information
Study from which key value was derived:
A 96-hour acute toxicity study was conducted to determine the ability of N,N-Didecyl-N-methylpoly(oxyethyl)ammonium Propionate to cause acute toxic effects to fish when tested in accordance with EPA OPPTS 850.1075. The test animals used were Bluegill (Lepomis macrochirus), exposed to the test material for 96 hours in a flow-through test system at concentrations of 0, 0.10, 0.20, 0.40, 0.80 and 1.6 mg a.s./l. (nominal concentrations: 0, 0.090, 0.19, 0.38, 0.77 and 1.4 mg a.s./l).
100% mortality was observed at the 0.77 and 1.4 mg a.s./L concentration groups and 5% mortality was recorded in the 0.38 mg a.s./L test group. No adverse effects were observed in any test group in the surviving fish. Based on concentration effect relationship observed, under the conditions of this study, the no-observed-effect concentration (NOEC) was found to be 0.19 mg/l.
Supporting study:
The acute toxicity of Bardap 26 (N,N-Didecyl-N-methylpoly(oxyethyl)ammonium Propionate) was determined in a 96 hour semi-static study conducted on Common carp (Cyprinus carpio) conducted in accordance with EC Method C1 and OECD Guideline No. 203. Dechlorinated tap water, run through an activated carbon filter, and partially softened was used as dilution water. 20 litre glass exposure vessels were used for each test concentration. Test vessels were pre-conditioned with the test solutions and covered to reduce evaporation and maintained at 21°C with a photoperiod of 16-hours light and 8-hours darkness.
The test material was tested at concentrations of 0, 0.22, 0.39, 0.7, 1.26, 2.24 mg a.s./l and water samples were taken from the control and all surviving test groups were taken at 0, 24, 48 and 96 hours for quantitative analysis. Duplicate samples were taken and stored frozen for further analysis if necessary. Mortality was the test parameter. 100% mortality was observed at 1.26 mg a.s./l and 2.24 mg a.s./l concentrations, with 70% mortality observed at 0.7 mg a.s./l. The LC50was determined to be 0.62 mg a.s./l, with the NOEC found to be 0.39 mg/L, based on the concentration effect relationship observed.
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