Registration Dossier
Registration Dossier
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EC number: 224-330-1 | CAS number: 4314-14-1
Endpoint summary
Administrative data
Description of key information
Additional information
The ready biodegradability of the test item Fat Yellow 3G was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days. The study was conducted according to OECD guideline 301 F. The test item concentration selected as appropriate was 28mg/L, corresponding to a ThOD of 51.2 mg O2/L (76.7 mg O2/L with nitrification) per test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation rate is therefore expressed as the percentage BOD (biological oxygen demand) and was calculated for each study day.
The mean oxygen depletion in the inoculum control was 9.2 mg O2/L on day 28.
In order to check the activity of the test system sodium benzoate was used as functional control. The pass level > 60 % was reached within 3 days. The biodegradation reached a maximum of 99 % degradation on day 26.
In the toxicity control containing both test and reference item 52 % degradation occurred within 14 days. After 28 days the biodegradation came to 55 %. The degradation of the reference item was not inhibited by the test item.
Both test item replicates did not reach the 10 % level (beginning of biodegradation) until test end. The mean biodegradation on day 28 was 4 %.
The validity criteria of the guideline are fulfilled.
The test item is classified as not readily biodegradable
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