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EC number: 265-064-6 | CAS number: 64741-62-4 A complex combination of hydrocarbons produced as the residual fraction from distillation of the products from a catalytic cracking process. It consists of hydrocarbons having carbon numbers predominantly greater than C20 and boiling above approximately 350°C (662°F). This stream is likely to contain 5 wt. % or more of 4- to 6-membered condensed ring aromatic hydrocarbons.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian cell study: DNA damage and/or repair
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Near-guideline, GLP-compliant study. Adequate for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- no guideline followed
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OTS 798.5915 (In Vivo Sister Chromatid Exchange Assay)
- Principles of method if other than guideline:
- Sister chromatid exchange in mouse bone marrow cells was determined 4 hr after a single i.p. injection of test substance.
- GLP compliance:
- yes
- Type of assay:
- sister chromatid exchange assay
Test material
- Reference substance name:
- 64741-62-4
- Cas Number:
- 64741-62-4
- IUPAC Name:
- 64741-62-4
- Test material form:
- other: Viscous hydrocarbon liquid
- Details on test material:
- Catalytic cracked clarified oil (CCCO), API 81-15, CAS No.
64741-62-4.
Dark brown viscous liquid
Data below taken from American Petroleum Institute (1985d). In-vivo sister chromatid exchange (SCE) assay. Catalytic cracked clarified oil, API Sample 81-15, CAS No. 64741-62-4. Testing laboratory: Microbiological Associates Inc., 5221 River Road, Bethesda, MD 20816, USA. Owner company: American Petroleum Institute, 2101 L Street, Northwest, Washington, DC 20037, USA. Study number: 32-32754. Report date: 1985-11-25.
Gravity API: 0.1
Specific gravity: 1.0753
Viscosity in SUS @ 210 °F: 56.1
Flash Point °F: 396
Sulfur wt %: 1.1
Pour Pt °F: 35
Asphaltenes % (MN 596): 4.2
Carbon residue wt %: 4.6
Ash wt %: 0.05
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Frederick Cancer Research Facility, Frederick, MD, USA.
- Age at study initiation: 9-10 wk
- Weight at study initiation: males 20-25 g, females 16-21 g
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- corn oil
- Details on exposure:
- Single i.p. injection in corn oil, 10 mg/Kg bw
- Duration of treatment / exposure:
- Bone marrow collected 20-22 hr post-treatment
- Frequency of treatment:
- Single treatment
- Post exposure period:
- 20-22 hr
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.4, 2.0 and 4.0 g/Kg bw
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5/sex/dose level
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide, 10 mg/Kg bw
Examinations
- Tissues and cell types examined:
- femural bone marrow cells
- Details of tissue and slide preparation:
- TREATMENT AND SAMPLING TIMES:
BRdU pellets implanted subcutaneously in lower abdomen 4 hr prior to treatment
Animals sacrificed 20-22 hr post-treatment.
Colchicine (1 mg/kg i.p.) given 2-4 hr prior to sacrifice.
DETAILS OF SLIDE PREPARATION:
Air-dried preparations of femural bone marrow cells stained with Hoechst 33258/Giemsa and mounted for microscopic examination.
METHOD OF ANALYSIS:
A minimum of 50 second-division metaphase spreads examined blind under oil immersion and scored for SCEs and chromosome number. The percentage cells in of first, second and third-division metaphase (100 cells) and the mitotic index (500 cells) were also determined. - Evaluation criteria:
- Results were considered positive if a statistically significant dose-related increase in SCE was present
- Statistics:
- Results analysed by ANOVA and the Students Range Test.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- positive
- Toxicity:
- yes
- Remarks:
- >4 g/Kg bw
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
- Dose range: 0, 1, 2, 4, 6, 8
- Mortality: 0/10, 0/10, 0/10, 1/10, 2/10, 9/10
An upper dose of 4 g/Kg bw selected (- low level of mortality, minimal effect on body weight)
Any other information on results incl. tables
Results were positive.
The test substance produced a small but significant dose-dependent increase in mouse bone marrow SCEs compared with the corn oil control.
The positive control group gave the expected results.
Treatment |
Mean (SD) SCE/cell/mouse |
|
male |
female |
|
0.0 |
5.43 (0.60) |
6.73 (0.68) |
0.4 |
7.43 (1.00) |
7.22 (1.17) |
3.0 |
8.43 (1.15) * |
8.06 (1.36) |
4.0 |
8.83 (1.60) * |
9.26 (0.95) * |
+ve control |
24.61 (7.39) * |
31.60 (7.24) * |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): positive
The test substance induced SCE in mouse bone marrow following single i.p. injection. - Executive summary:
The potential of catalytic cracked clarified oil to induce sister chromatid exchanges in mouse bone marrow was investigated in vivo in a GLP-compliant study. Animals received a single i.p. treatment of 0 (corn oil), 0.4, 2.0 or 4.0 g test substance /Kg bw/d 20-22 hr prior to sacrifice, with positive controls given a single i.p. treatment of 0 or 10 mg/Kg cyclophosphamide. Microscopic examination of a minimum of 50 second-division metaphase spreads under oil immersion revealed a significant and dose-related increase in SCEs per metaphase in both sexes; a satisfactory response was obtained in the positive control group.
The results demonstrated that test substance had a clear potential to induce SCEs in femoral bone marrow in vivo.
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