Manganese

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Peer reviewed journal

Justification for type of information:

Data is from Peer reviewed journal.

Qualifier:

according to guideline

Guideline:

ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate)

GLP compliance:

not specified

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM:
- Age at study initiation: 2 months old
-Other: organism stored in commercial worm bedding with worm food
ACCLIMATION
- Acclimation period: one week

Study type:

laboratory study

Substrate type:

natural soil

Total exposure duration:

16 d

Test temperature:

20°C

Details on test conditions:

TEST SYSTEM
- Test vessel: polypropylene wide-mouth jars with loose fitting lids
- No. of organisms per vessel: Ten adult
- Aeration: The lids of the jars were punched with holes to allow ventilation and oxygen supply

Reference substance (positive control):

yes

Remarks:

Natural soil

Key result

Duration:

16 d

Dose descriptor:

LOEC

Effect conc.:

50 other: %

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Biomass

Duration:

16 d

Dose descriptor:

LOEC

Effect conc.:

2 other: Stimulant unit

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Biomass

Details on results:

The 16 days LOEC value was determined to be 50% for toxicity and 2 for Stimulant effect.

Reported statistics and error estimates:

Statistical analysis was carried out on a microcomputer using the TOXSTAT 3.0 software.

Validity criteria fulfilled:

not specified

Conclusions:

The 16 days LOEC value was determined to be 50% for toxicity and 2 for Stimulant effect.

Executive summary:

The study was conducted to determine toxicity of test chemical to soil macro-organism in accordance with ISO (1993b) methodology. The test was performed on 2 months oldEisenia foetida which were stored in commercial worm bedding with worm food. Test was performed for 16 days in polypropylene wide-mouth jars with loose fitting lids at a temperature of 20°C. Aeration was achieved by punching the lids of jar with holes to allow ventilation and oxygen supply.Ten adult earthworms were placed into 600 g(dry weight) of natural soil which were used as control or soil: sludge mixtures of different proportions i.e., 50; 25; 12.5; and 6.2% which previously hydrated to 25% water-holding capacity in 1000-ml polypropylene wide-mouth jars with loose fitting lids to minimize water evaporation and animal escape. The 16 days LOEC value was determined to be 50% for toxicity and 2 for Stimulant effect. E. foetida showed a biomass increase when exposed to the stabilized textile sludge.

Description of key information

The 16 days LOEC value was determined to be 50% for toxicity and 2 for Stimulant effect.

Additional information

Following study includes experimental study for the target chemical to observe the toxicity of test chemical to soil macro-organisms as follows:

The study was conducted to determine toxicity of test chemical to soil macro-organism in accordance with ISO (1993b) methodology. The test was performed on 2 months oldEisenia foetida which were stored in commercial worm bedding with worm food. Test was performed for 16 days in polypropylene wide-mouth jars with loose fitting lids at a temperature of 20°C. Aeration was achieved by punching the lids of jar with holes to allow ventilation and oxygen supply.Ten adult earthworms were placed into 600 g(dry weight) of natural soil which were used as control or soil: sludge mixtures of different proportions i.e., 50; 25; 12.5; and 6.2% which previously hydrated to 25% water-holding capacity in 1000-ml polypropylene wide-mouth jars with loose fitting lids to minimize water evaporation and animal escape. The 16 days LOEC value was determined to be 50% for toxicity and 2 for Stimulant effect. E. foetida showed a biomass increase when exposed to the stabilized textile sludge.