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EC number: 248-363-6 | CAS number: 27247-96-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- As mortality occurred at the limit test concentration (LS) on day 1, sampling was stopped and a full definitive test was undertaken.
The sampling were performed by the Principal Investigator then sent to the test facility for analysis. The samples were analysed within 5 days after sampling according to the validated method.
Test item was stored at room temperature in poison cabinet. - Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Conditions of housing:
Temperature: at 23°C ± 2°C.
Light: light/dark cycle 16/8 hours.
Freshwater (see chapter 12.1.1.).
Forced aeration to keep the dissolved oxygen concentration at or above 80% of the saturation value at the selected temperature.
Feeding : twice daily with tropical fish chow.
Acclimation period:
All fish were held in the laboratory where the study phase was performed seven days before the start of the range finding test. During the acclimation period, fish were subjected to the same conditions of housing (i.e. temperature, photoperiod, oxygen content and feeding). The feeding was stopped 24 hours before the start of the test.
One fish for four hundred received is dead during the last week before the test, the batch was agreed to be conformed.
Mortality was less than 5% for the 7 first days.
Sensibility of the batch was checked by a toxicity test with reference item (K2Cr2O7) at tow concentrations (200 and 400 mg/L). We have observed twenty nine percent mortality with 200 mg/L condition and one hundred percent mortality with 400 mg/L condition after 24h exposure. So, LC50(24h) K2Cr2O7 is between 200 and 400mg/L. The batch was agreed to be conformed.
LC50(24h) K2Cr2O7 was between 200 and 400mg/L. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- T0 (08/23/2010)= 23 °F
T96h (08/27/2010) = 22.3 °F - Test temperature:
- 23°C ± 2°C
- pH:
- From 7.05 to 8.12
- Dissolved oxygen:
- From 35.70 to 98.60
- Nominal and measured concentrations:
- 0.92, 1.46, 2.34, 3.75 and 6 mg/L
- Details on test conditions:
- The aim of this study phase was to perform acute toxicity test to the zebra fish under semi-static conditions.
Tow essay were performed, one range finding test and one definitive test. In the definitive test fishes were exposed to five concentrations (0.92 ; 1.46 ; 2.34 ; 3.75 and 6 mg/l nominal concentration). In each condition, before and after renewal, sample were taken for essay item extraction in toluene and sent to CIT for analysis. At the beginning of the test seven fish were introduced in each vessel felled with essay solution and caped to avoid to important degradation of the essay item. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.52 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Validity criteria
All validity criteria of the study were respected.
Chemical analysis
Measured concentrations in the definitive test solutions at 0.92 and 1.46 mg/L were below the limit of quantification (< 0.8 mg/L) or detection (< 0.2 mg/L).
Measured concentrations in the definitive test solutions at 2.34, 3.75 and 6.00 mg/L dropped below 80% of the corresponding nominal values during the study, therefore the toxicity results were based on geometric means of measured concentrations during the corresponding exposure period, i.e. 96 hours.
Mortality
At T96 hours, mortality was 100% at the nominal concentration of 6 mg/L and 0% in all other tested concentrations. Hence, the 96-hour LC50 corresponds to the geometric mean of the highest concentration causing no mortality (3.75 mg/L nominal) and the lowest concentration producing 100% mortality (6 mg/L nominal). These concentrations were also used as confidence limits. - Sublethal observations / clinical signs:
At T96 hours,mortality was 100% at the nominal concentration of 6 mg/L and 0% in all other tested concentrations. Hence, the96-hourLC50corresponds to the geometric mean of the highest concentration causing no mortality (3.75 mg/L nominal) and the lowest concentration producing 100% mortality(6 mg/L nominal). These concentrations were also used as confidence limits.
The results and conclusions are summarized in the following tables:
Group
Control
2-Ethylhexyl nitrate
Nominal concentration (mg/L)
0
0.92
1.46
2.34
3.75
6.00
Measured concentration (mg/L)
Geometric mean 0-96 hours
0
ND
ND
1.04
1.52
2.64
Effects
Mortality (%) - 96 hours
0
0
0
0
0
100
Endpoints (mg/L)(1)
LC50- 96 hours
2.00
(1.52 - 2.64)
LC0- 96 hours
1.52
NOEC - 96 hours
1.52
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a 96-hour semi-static acute toxicity test with Danio rerio, the LC50 of 2-Ethylhexyl nitrate was 2.00 mg/L.
The No Observed Effect Concentration NOEC was 1.52 mg/L.
Reference
Description of key information
In a 96-hour semi-static acute toxicity test with Danio rerio, the LC50 of 2-Ethylhexyl nitrate was 2.00 mg/L.
The No Observed Effect Concentration NOEC was 1.52 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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