Calcium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

- Storage condition of test material: At room temperature at about 20 °C, away from direct sunlight

Analytical monitoring:

yes

Details on sampling:

- Sampling method: duplicates of both concentrations after 0 48 and 96 hours
- Sample storage conditions before analysis: deep-frozen (at about -20 °C) immediately after sampling

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: due to the limited water solubility of the product, containing sparingly soluble components, a water accommodated fraction (WAF) was prepared according to OECD guidence documet No. 23. A dispersion of 100 mg/L was prepared by mixing 700.3 mg of the test item into 7000 ml of test water. The dispersion was stirred in the dark for 3 hours. After the stirring period of 3 hours, the dispersion was filtered through a membrane filter

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: RCC laboratories
- Length at study initiation: 3.0 ± 0.2 cm
- Weight at study initiation: 0.37 ± 0.03 g
- Feeding during test: none

ACCLIMATION
- Acclimation period: one week
- Acclimation conditions: same as test
- Type and amount of food: commercial fish diet (Tetra Min® Hauptfutter, supplied by TETRA-Werke, 49304 Melle; Germany)
- Health during acclimation: During the last two weeks prior to the test no fish died in the test fish batch and all fish were healthy

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

1.25 mmol/L

Test temperature:

22 °C

pH:

7.3 - 7.4

Dissolved oxygen:

8.0 - 8.2 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: control, 100 mg/L
- Mean measured concentrations: < detection limit, 0.01 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel material, fill volume: glass aquarium, 6 L
- Aeration: slightly
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.5 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted test water
- Alkalinity: 0.4 mmol/L
- Ca / Mg ratio: 4:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature, ph, oxygen content after 0, 24, 48, 72, 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hour light : 8 hour darkness ( 30 min transition period)
- Light intensity: 50 to 500 lux

EFFECT PARAMETERS MEASURED: mortality and visible abnormalities after 3, 24, 48, 72, 96 hours

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: no effects observed up to the limit of water solubility

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Appearance of the test medium: no remarkable observations, clear test medium. No toxic effects occur within the range of solubility.

Validity criteria fulfilled:

yes

Conclusions:

The test item is with high probability not acutely harmful to fish.

Description of key information

The test item is with high probability not acutely harmful to fish.

Additional information

One study on the acute toxicity of the substance to fish are available. The test system was set up according to OECD guideline 203 (RCC Ltd, 2007). Danio rerio were exposed to the substance for 96 hours in a static test system. At test termination no effects were observed up to the limit of water solubility (at a nominal loading rate of 100 mg/l).