Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-532-0 | CAS number: 96-76-4
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (3h contact time) >10 mg/L (OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 mg/L
Additional information
Phenol 2,4 -bis(1,1-dimethylethyl) was evaluated for its toxicity on a mixed population of activated sewage sludge microorganisms. EC50 value (3h contact time) for test substance was > 10 mg/l. The test substance is toxic to microorganisms.
Test method:
The method used followed that described in the OECD Guidelines for testing of Chemicals / Section 2: Effects on Biotic Systems, Test No. 209 "Activated Sludge, Respiration Inhibition Test". Deviations are in the number and values of concentrations (this is not expected to compromise the quality of the study results).3,5 -dichlorophenol was the reference substance at 3.2 and 32 mg/l. O2 consumption rates were measured after 3 h contact time at concentrations of 10, 100 and 1000 mg/l of test substance in the range finding study, and 10 mg/l for the 3 replicates in the definitive study. Variation between controls was ± 5% in the range finding study and ± 3% in the definitive study. EC50 value (3h contact time) for reference substance was 10 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
