Ethanol, 2-amino-, reaction products with ammonia, 1-piperazineethanamine fraction

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure according to national standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German industrial standard test guideline DIN 38 412, part L15.

GLP compliance:

no

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): hydroxyethylpiperazine
- Analytical purity: 98 - 99%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The doses were chosen based on a range finding study.
The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 8.1 cm (range: 7.2 - 9.3)
- Weight at study initiation: 5.3 g (range: 3.6 - 8.3)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day


ACCLIMATION
- Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature: 20 ± 1°C
Acclimation to test water and test temperature: 3 days

- Type and amount of food: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: ad libitum.

HOUSING
- Housing water: charcoal filtered water in continuous flow-through
- Aeration: oilfree air
- Light regime: 16 : 8 hours light : dark
- Hardness: 2.5 mM
- Oxygen concentration: > 60 % of maximum saturation
- pH: about 8.0
- Water temperature: 15 - 20 °C
- Duration of housing: about 3 months
- Medical treatment: Twice with 0.05 mg/L Malachitegreen chloride and once with 10 mg/L tetracyclinhydrochlorid
- Feeding: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 0.03 %

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: 2.5 mmol/L
Acid capacity: 0.8 mM

Test temperature:

20 - 21 °C

pH:

7.2 - 10.9

Dissolved oxygen:

7.2 - 8.9 mg/L

Nominal and measured concentrations:

Nominal concentrations: 100, 215, 464, 1000, 2150, 4640 mg/L. Additionally: Neutralised samples at 4640 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 5.3 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Na/K ratio: 10:1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

Reference substance (positive control):

yes

Remarks:

Chloracetamid

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 464 - < 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: apathy, narcotic-like state, tumbling

Results with reference substance (positive control):

- Results with reference substance valid? yes
- LC50: ca. 32 mg/L (48 h)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Cumulative mortality

Nominal conc.(mg/l)	Number of fish	Mortality (cumulated) after
		1 h	4 h	24 h	48 h	72 h	96 h
0	10	0	0	0	0	0	0
100	10	0	0	0	0	0	0
215	10	0	0	0	0	0	0
464	10	0	0	0	0	0	0
1000	10	0	0	0	5	8	8
2150	10	0	3	10	10	10	10
4640	10	7	10	10	10	10	10
4640*	10	0	0	0	0	0	0

* = pH adjusted

observed symptoms

Nominal conc.(mg/l)	Symptoms after
	1 h	4 h	24 h	48 h	72 h	96 h
0
100
215
464
1000			AT	A	A	A
2150	A	ANT
4640	N
4640*

*: pH-adjusted; A: Apathy, N: Narcotic like state, T: Tumbling

pH values

Nominal conc.(mg/l)	pH values after
	1 h	24 h	48 h	72 h	96 h
0	8.2	8.0	7.7	7.6	7.7
100	9.4	8.2	7.7	7.7	7.7
215	9.7	9.0	7.8	7.7	7.7
464	10.0	9.3	8.3	8.0	8.0
1000	10.3	9.7	8.3	8.8	8.7
2150	10.6	10.2
4640	10.9
4640*	8.0	7.8	7.2	7.3	7.3

*: pH-adjusted

oxygen content

Nominal conc.(mg/l)	Oxygen concentrations [mg O2/L] after
	1 h	24 h	48 h	72 h	96 h
0	7.9	7.4	8.0	7.3	7.1
100	7.7	8.2	8.2	7.8	7.5
215	8.2	7.2	8.6	8.9	8.5
464	8.3	7.7	8.8	8.7	8.4
1000	8.4	8.2	7.8	7.9	8.3
2150	8.6	8.8
4640	8.8
4640*	8.6	7.7	7.7	8.3	8.9

*: pH adjusted

Applicant's summary and conclusion

Validity criteria fulfilled:

yes