Methyl cyanoacetate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Methyl cyanoacetate

EC Number:

203-288-8

EC Name:

Methyl cyanoacetate

Cas Number:

105-34-0

Molecular formula:

C4H5NO2

IUPAC Name:

methyl 2-cyanoacetate

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp (cyprinus carpio)
- Length at study initiation (length definition, mean, range and SD): mean 2.8 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14 days
- Type and amount of food: the daily of feed is approx. 2% of the body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): The mortality 7 days before test began was < 5%

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

14° dH

Test temperature:

min. = 19.3°C / max. 21.1°C
mean value = 20.1°C

pH:

4.4 - 8.3 Discrepancy of the pH from desired value (pH 8 ± 1) is an effect of the product in water

Dissolved oxygen:

97-102 % saturation

Nominal and measured concentrations:

nominal concentrations: 60, 105, 185, 325, 570 and 1000 mg/l
The measured concentrations for stability control after 24 h from the 1 st batch did not deviate by more than 20% from the freshly prepared test
solutions. It can be concluded that the test item (or possible hydrolysis products) was present over the whole exposure time. Therefore, the
biological values were calculated with the nominal concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 l glass tank
- Aeration: continously in the test tanks
- No. of organisms per vessel: 10 animals per test tank, each at approx. 0.70 g
- water composition:
sum of Ca 2+ / Mg 2+ ions = 2.5 mmol
proportion of Ca 2+ / Mg 2+ ions = 4:1
proportion of Na+ / K+ ions = 10:1

OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle was 12 to 12 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

245 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

139 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

139 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

123 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

60 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

185 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The highest concentration at which no mortality (<10%) after 96 h exposure occurred, was 96h LCo = 60 mg/I.
The lowest concentration at which 100 % mortality after 96 h exposure occurred, was 96h LC100 = 185 mgll.
The concentrations are based on the test item.