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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No guideline was followed and no GLP. However, the test method is similar to OECD 401.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Principles of method if other than guideline:
No guideline was followed but the test method was similar to OECD 401.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl chloroacetate
EC Number:
202-501-1
EC Name:
Methyl chloroacetate
Cas Number:
96-34-4
Molecular formula:
C3H5ClO2
IUPAC Name:
methyl 2-chloroacetate
Details on test material:
- Name of test material (as cited in study report): monochloressigsäuremethylester
- Physical state: colourless liquid

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 219 ± 24.69 g
- Diet (e.g. ad libitum): Altromin 1324 (Altromin GmbH, Lage/Lippe), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: sesame oil
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 0.5 g substance/100 ml vehicle
Doses:
50, 80, 100, 125, 200 and 315 mg/kg bw
No. of animals per sex per dose:
10 females per dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: body weight was recorded at 0, 7 and 14 days
- Other examinations performed: clinical signs, necropsy.
Statistics:
Probit analysis

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
107 mg/kg bw
95% CL:
97.2 - 121
Mortality:
Mortality was observed at 100, 125, 200 and 315 mg/kg bw.
Clinical signs:
other: Passivity, disturbances of equilibrium, prone position, piloerection, diarrhea, laboured respiration.
Gross pathology:
Liver, spleen and kidneys deep brown coloured and strongly supplied with blood, red ovaries.

Any other information on results incl. tables

Table 7.2.1 -1: Mortality

Dose (mg/kg bw)

Mortality

50

0/10

80

0/10

100

4/10

125

8/10

200

10/10

315

10/10

 

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category III
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The oral LD50 (females) was 107 mg/kg bw with a 95% confidence interval between 97.2 and 121 mg/kg bw.
Executive summary:

The test substance methyl chloroacetate was administered to groups of female Wistar rats by oral gavage at the doses of 50, 80, 100, 125, 200 and 315 mg/kg body weight. Animals were subjected to daily observations and weekly determinations of body weight. Macroscopic examinations were performed on the animals found dead. Mortality was observed at 100, 125, 200 and 315 mg/kg bw. The oral LD50 was 107 mg/kg bw with a 95% confidence interval between 97.2 and 121 mg/kg bw.