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Diss Factsheets
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EC number: 227-177-9 | CAS number: 5698-98-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- other: Experimental study with acrylic acid (structural analogue) which is used for read-across (see attached read across justification document in IUCLID section 13)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Sufficiently documented and scientifically acceptable. Justification for read-across: similar chemical structure (see Cemical Safety Report)
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- yes
- Remarks:
- no data on quality assurance and data storage
- GLP compliance:
- not specified
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Acrylic acid
- EC Number:
- 201-177-9
- EC Name:
- Acrylic acid
- Cas Number:
- 79-10-7
- IUPAC Name:
- acrylic acid
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): acrylic acid
- Substance type: organic substance
- Physical state: liquid
- Analytical purity: >99.8%
- supplier: Hoechst Celanese Company (Sommerville, NJ, USA)
Constituent 1
Method
- Target gene:
- HGPRT locus
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- CHO-K1-BH4 cells obtained from Oak Ridge National Laboratory, USA
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S-9
- Test concentrations with justification for top dose:
- 0.3, 0.6, 1.0, 1.5, 1.9, 2.4, 2.8 µl/ml
- Vehicle / solvent:
- phosphate buffer
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation); preincubation; in suspension; as impregnation on paper disk
DURATION
- Preincubation period:18-25 h
- Exposure duration: 5 h
- Expression time (cells in growth medium): 7-9 day
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED: 100 cell/60 mm dish
DETERMINATION OF CYTOTOXICITY
- Method: cloning efficiency - Evaluation criteria:
- toxicity (% survival), total mutant colonies, mutants/ 10 exp 6 clonable cells
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- concentrations of 2.4 µl/ml and higher caused cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: adjusted to pH 7.5
- Effects of osmolality: osmolarity was measured and dosing solutions of less than 400 mosmol/kg were used.
ADDITIONAL INFORMATION ON CYTOTOXICITY: concentrations of 2.4 µl/ml and higher caused cytotoxicity, concentrations of 2.8 µl/ml were too cytotoxic for evaluation (2% survival).
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Acrylic acid was assessed as not mutagenic in the CHO/HGPRT mutation assay. - Executive summary:
Acrylic acid (>99.8% pure) was tested for gene mutations in the CHO/HGPRT assay.
After incubation of 5 x 10 exp 5 cells/25 -cm² at 37 °C for 18 -24 h the test compound was added to the culture medium. The pH was monitored and adjusted and the osmalarity was measured. Triplicate cultures with and without metabolic activation (S9) were exposed for 5 h at 37 °C. At the end of the exposure period the medium was removed, the cells washed and cultured for an additional 18 -24 h. After adding additional cell for assessing the cytotoxicity the incubation was continued for 7 -9 days. At the end of this expression period a selection for thioguanine resistant mutants was performed.
Concentrations of 2.4 µl/ml and higher caused cytotoxicity, concentrations of 2.8 µl/ml were too cytotoxic for evaluation (2% survival).
The mutation frequencies for all treatments with acrylic acid were within the normal variation of the background. Consequently, acrylic acid was assessed as not mutagenic in the CHO/HGPRT mutation assay.
This result can be extrapolated to magnesium acrylate.
Please refer to the justification for read across in the Chemical Safety Report.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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