Tricobalt dicitrate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given.

Data source

Reference

Reference Type:

publication

Title:

Acute oral toxicity of inorganic cobalt compounds in rats.

Author:

Speijers, G.J.A. et al.

Year:

1982

Bibliographic source:

Food and chemical toxicology. 20: 311-314

Materials and methods

Principles of method if other than guideline:

The test compound was administered to rats by gastric intubation in a single dose. Signs of reactions and deaths were recorded for 10 days. No pathologic or histopathologic examinations were performed.

GLP compliance:

not specified

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

10026-24-1

EC Number:

600-050-9

Cas Number:

10026-24-1

IUPAC Name:

10026-24-1

Constituent 2

Reference substance name:

cobalt(II) sulphate heptahydrate

IUPAC Name:

cobalt(II) sulphate heptahydrate

Details on test material:

- Name of test material (as cited in study report): cobalt(II) sulphate heptahydrate
- Molecular formula (if other than submission substance): CoSO4*7H2O
- Molecular weight (if other than submission substance): 281 g/mol
- Analytical purity: 99%

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: from the colony of the Animal Breeding Unit, National Institute of Public Health, Bilthoven, Netherlands
- Weight at study initiation: 100 g
- Housing: The animals were housed singly or in pairs of the same sex and dose group in wire cages.
- Diet (e.g. ad libitum): semi-purified diet, Muracon-SSP Tox (Trouw Ltd, Putten)
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22-25
- Humidity (%): 35-55

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

450, 675, 1000, 1500, 2250 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 10 days
- Necropsy of survivors performed: no
- Other examinations performed: The rectal temperature was measured in all surviving animals 1.5, 24 and 48 hours after administration.

Statistics:

The oral LD50 values (for male and female rats combined) were calculated according to the method of maximum likelihood of Finney (1971).

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

768 mg/kg bw

95% CL:

594 - 995

Remarks on result:

other: CoSO4*7H2O

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

679 mg/kg bw

Remarks on result:

other: CoC6H6O7 (recalculated value from CoSO4*7H2O)

Mortality:

No mortality data were reported.

Clinical signs:

other: The highest dose level (2250 mg/kg bw) caused sedation and diarrhoea. Rats showed tremors and convulsions prior to death. In all animals, decrease in body temperature was recorded, varying from 2.5 to 7.5 °C. The temperature reduction was time- and dose-r

Any other information on results incl. tables

LD50 (CoSO4*7H2O): 768 mg/kg bw (594 -995)

LD50 (CoSO4): 424 mg/kg bw (recalculated value from CoSO4*7H2O)

LD50 (Co(II) ion): 161 mg/kg bw (recalculated value from CoSO4*7H2O)

LD50 (CoC6H6O7): 679 mg/kg bw (recalculated value from CoSO4*7H2O)