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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
carcinogenicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study does not meet current standards (small number of animals, limited scope of examinations) and is not GLP compliant. In the OECD SIDS on 2-Naphthol (2002) a similar reliability was assigned.

Data source

Reference
Reference Type:
publication
Title:
The tumor-promoting action of phenol and related compounds for mouse skin.
Author:
Boutwell RK and Bosch DK
Year:
1959
Bibliographic source:
Cancer Res. 19, 413-424

Materials and methods

Test guideline
Guideline:
other: Initiation-promotion-study
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-naphthol
EC Number:
205-182-7
EC Name:
2-naphthol
Cas Number:
135-19-3
Molecular formula:
C10H8O
IUPAC Name:
2-naphthol
Details on test material:
purity not stated

Test animals

Species:
mouse
Strain:
other: different strains : CAF1, C3H, Holtzman
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: (a) those from Arthur Sutter of Springfield, Mo., (b) those from Holtzman Rat Co. of Madison, Wisconsin, (c) our own colony of mice derived from Sutter mice, and (d) the CgH and CAF1 mice obtained from the Roscoe B. Jackson Laboratory, Bar Harbor, Maine.
- Age at study initiation: 2-3 months
- Housing: housed in screen-bottomed metal cages in air-conditioned animal rooms
- Diet and water (e.g. ad libitum): Water and a diet of Purina Laboratory Chow were given ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature: 75°F

Administration / exposure

Route of administration:
dermal
Vehicle:
other: acetone or ethanol
Duration of treatment / exposure:
12, 21 weeks
Frequency of treatment:
two times per week
Post exposure period:
not specified
Doses / concentrations
Remarks:
Doses / Concentrations:
25 µl of a 20 % solution in acetone (12 week study) or ethanol (21 week study)
Basis:

No. of animals per sex per dose:
36 females
Control animals:
other: solvent control and positive control (croton oil)
Details on study design:
Initiation with 0.3 % DMBA (75 ug) in acetone or benzene. 36 female mice from four sources, age at study begin: 2-3 months. One week after initiation, 5 mg of 2-naphthol (in benzene or acetone) was applied twice per week for 12 or 21 weeks. Mice were examined weekly for tumors.

Results and discussion

Results of examinations

Details on results:
12-week study: 33 of 36 animals survived; 3 % of the animals developed papillomas. None of the animals had skin carcinoma. 21-week study: 21 out of 24 animals survived; none of the animals showed skin papilloma or skin carcinoma. 2-Naphthol had no tumour promoting activity on mouse skin.

Applicant's summary and conclusion

Conclusions:
A Initiation-promotion-study on 2-naphthol was performed on different strains of mice from 4 sources. A 12, 21 week study showed that 2-Naphthol had no tumour promoting activity on mouse skin.