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EC number: 938-147-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-01-10 to 2012-02-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- not specified
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: No vehicle, directly weighing of the stock solution (10 g a.i./L)
- Eluate: Synthetic waste water acc. to OECD 209
- Differential loading: 1000 - 580 - 320 - 180 - 100 mg/L
- Controls: Test medium without test or reference substance. 6 replicates: Triplicates at the beginning of the test + triplicates at the end of the test - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at sarstedt (Municipal sewage treatment plant of 31157 Sarstedt, Germany)
- Pretreatment: Washed twice with autoclaved tap water and adjusted to a dry weight concentration of 3 g/L +/- 10 %
- Dry sludge concentration: 3.05 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Not determined
- Test temperature:
- 20-21 °C
- pH:
- 7.88 (activated sludge)
7.37 (synthetic waste water) - Salinity:
- None, freshwater
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 3
- Composition of test medium:
250 mL inoculum
16 mL synthetic waste water
234 mL autoclaved tap water
Test or reference item concentration (directly weighed into each test vessel)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control. reference item and test item replicate was determined by means of oximeter immediately after transferring the solution to the measuring cell and the oxygen depletion was recorded for at least 3 minutes on a flat bed recorder.
TEST CONCENTRATIONS
- Range finding study
Inhibition of Respiration (Preliminary Test)
Nominal Test
Concentration
[mg a.i./L] Inhibition[%]
10 2
100 -15
1000 89 - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 372 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 347-398 (95% CI)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 791 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 733-847 (95% CI)
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 129 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the active ingredient is 320 mg/L, the EC10 is 372 mg/L and the EC50 791 mg/L to activated sludge of a municipal sewage treatment plant.
- Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Hostapon SG (batch number: ESD 0006364) from 2012-01-10 to 2012-02-07, with the experimental phase on 2012-02-07 at Dr.U.Noack-Laboratorien, D-31157 Sarstedt. Test system was activated sludge of the municipal treatment plant of D-31137 Hildesheim. The test was carried out under static conditions with the nominal active ingredient concentrations 100 - 180 - 320 - 580 - 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from -33 % to 62 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 129 mg/L.
Table 1: EC-Values and NOEC of Hostapon SG (a.i.)
Effect values (nominal a.i.)
Confidence interval
[mg/L]
P = 95 [%]
[mg/L]
NOEC
320
–
EC10
372
347 – 398
EC20
452
422 – 486
EC50
791
733 – 847
EC80
> 1000
n.d.
Reference
EC-Values and NOEC ofHostapon SG (a.i.)
Effect values (nominal a.i.) |
Confidence interval |
|
[mg/L] |
P = 95 [%] [mg/L] |
|
NOEC |
320 |
– |
EC10 |
372 |
347 – 398 |
EC20 |
452 |
422 – 486 |
EC50 |
791 |
733 – 847 |
EC80 |
> 1000 |
n.d. |
Oxygen Uptake Rates, Specific Respiration Rates and Inhibition of the Respiration of the Control and Reference Item Concentrations
Reference Item Concentration |
|
Oxygen Uptake Rate |
Specific Respiration Rate |
|
Mean Inhibition |
Control |
1 |
33.6 |
22.0 |
– |
– |
2 |
34.0 |
22.3 |
|||
3 |
33.2 |
21.8 |
|||
4 |
36.0 |
23.6 |
|||
5 |
34.4 |
22.6 |
|||
6 |
33.6 |
22.0 |
|||
58 |
1 |
29.2 |
14 |
13 |
|
2 |
31.2 |
9 |
|||
3 |
28.4 |
17 |
|||
100 |
1 |
21.6 |
37 |
38 |
|
2 |
21.6 |
37 |
|||
3 |
20.0 |
41 |
|||
180 |
1 |
12.4 |
64 |
65 |
|
2 |
12.0 |
65 |
|||
3 |
11.6 |
66 |
Oxygen Uptake Rates, Specific Respiration Rates and Inhibition of the Respiration of the Test Item Concentrations
|
|
Oxygen Uptake Rate |
|
Mean Inhibition |
100 |
1 |
42.0 |
-23 |
-33 |
2 |
45.6 |
-34 |
||
3 |
48.0 |
-41 |
||
4 |
44.0 |
-29 |
||
5 |
46.8 |
-37 |
||
180 |
1 |
43.2 |
-27 |
-22 |
2 |
40.8 |
-20 |
||
3 |
41.6 |
-22 |
||
4 |
43.6 |
-28 |
||
5 |
38.0 |
-11 |
||
320 |
1 |
32.8 |
4 |
5 |
2 |
32.4 |
5 |
||
3 |
33.6 |
1 |
||
4 |
32.0 |
6 |
||
5 |
31.2 |
9 |
||
580 |
1 |
22.8 |
33 |
32 |
2 |
23.6 |
31 |
||
3 |
22.4 |
34 |
||
4 |
22.8 |
33 |
||
5 |
24.0 |
30 |
||
1000 |
1 |
13.6 |
60 |
62 |
2 |
13.6 |
60 |
||
3 |
12.8 |
62 |
||
4 |
12.4 |
64 |
||
5 |
12.0 |
65 |
Description of key information
The NOEC of the active ingredient is 320 mg/L, the EC10 is 372 mg/L and the EC50 791 mg/L to activated sludge of a municipal sewage treatment plant.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 791 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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