3-methylbut-3-en-1-ol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1698-10-29 and 1968-11-058

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment; pre-GLP study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

BASF Test (internal standard procedure). In principle, the methods described by OECD TG 401 were used.

GLP compliance:

no

Remarks:

pre-GLP study

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-3-en-1-ol

EC Number:

212-110-8

EC Name:

3-methylbut-3-en-1-ol

Cas Number:

763-32-6

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-3-en-1-ol

Details on test material:

- Name of test material (as cited in study report): 3-methyl-3-buten-1-ol
- Analytical purity: 99%
No further data.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
male and female Sprague-Dawley rats
- Source: Gassner
- Weight at study initiation: body weight range: males: 138 - 190 g; females: 124 - 158 g
No further data

ENVIRONMENTAL CONDITIONS: no data

IN-LIFE DATES: no data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: aqueous Traganth (emulsion)

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 2 - 30 % (v/v)

Doses:

ca. 170, 1360, 2720, 5440 mg/kg bw (200, 1600, 3200, 6400 ml/kg bw)

No. of animals per sex per dose:

10 males and 10 females per dose group

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: Group-wise documentation of clinical signs was performed over the 7-day observation period. Body weight was determined before the study only, as it was needed for determination of the dose.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology

Statistics:

no data

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 440 mg/kg bw

Mortality:

Four out of 20 rats of group 1 (6400 ml/kg) died; total mortality rate was 2/20, 3/20, and 4/20 by 24 h, 48 h and 7 days post dose, respectively. No deaths were observed at lower doses. See also Table 2.

Clinical signs:

other: Clinical symptoms were observed in all dose groups; incidence and severity showed a trend to dose-relationship. Clinical symptoms comprised marked staggering, prone/lateral position with extended extremities, narcosis-like state, dyspnea, chewing movement

Gross pathology:

Pathology of the decedents revealed distended gastro-intestinal tract (1 animal), possible renal changes (1 animal), and putrefaction. Signs of cannibalism were noted for 2 animals.
No pathologic findings were noted in survivors that had been sacrificed at the end of the observation period.

Any other information on results incl. tables

Table 2: mortality

 

group	number of animals	concentration in vehicle [% (v/v)]	applied dose		mortality rate within
			[ml/kg bw	[mg/kg bw]	1 h	24 h	48 h	7 d
1	20	30	6400	5440	0/20	2/20	3/20	4/20
2	20	30	3200	2720	0/20	0/20	0/20	0/20
3	20	20	1600	1360	0/20	0/20	0/20	0/20
4	20	2	200	170	0/20	0/20	0/20	0/20

 

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Remarks:

Migrated information

Conclusions:

3-Methyl-3-buten-1-ol was practically nontoxic in rats after ingestion.