Registration Dossier
Registration Dossier
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EC number: 200-598-5 | CAS number: 64-86-8
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- publication
- Title:
- Origin of aneuploidy in relation to disturbances of cell-cycle progression. II. Cytogenetic analysis of various parameters in mouse bone marrow cells after colchicine or hydroquinone treatment.
- Author:
- Pacchierotti F, Bassani B, Leopardi P, Zijno A
- Year:
- 1 991
- Bibliographic source:
- Mutagenesis, 6, 307-311.
Materials and methods
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Colchicine
- EC Number:
- 200-598-5
- EC Name:
- Colchicine
- Cas Number:
- 64-86-8
- Molecular formula:
- C22H25NO6
- IUPAC Name:
- colchicine
- Reference substance name:
- N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]acetamide
- IUPAC Name:
- N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]acetamide
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- other: C57BL; C3H
- Sex:
- male
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- H2O bidest.
- Duration of treatment / exposure:
- 18h and 24h after a single administration
- Frequency of treatment:
- single administration
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
1 mg/kg b.w.
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
3 mg/kg b.w.
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
6 mg/kg b.w.
Basis:
nominal conc.
- No. of animals per sex per dose:
- Male mice (4-6 per group)
Examinations
- Tissues and cell types examined:
- Bone marrow
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- positive
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): positive
Positive. There were clear dose-related increases in the frequency of micronuclei. However, high levels of cell toxicity (block in mitosis and a marked decrease in %PCE) were also seen.
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