1,3,5-triisopropylbenzene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,3,5-triisopropylbenzene

EC Number:

211-941-3

EC Name:

1,3,5-triisopropylbenzene

Cas Number:

717-74-8

Molecular formula:

C15H24

IUPAC Name:

1,3,5-tris(propan-2-yl)benzene

Test animals

Species:

guinea pig

Strain:

Hartley

Sex:

male/female

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

A limit dose of 20 ml/kg was applied to 5 male and 5 female guinea pigs The backs of the guinea pigs were clipped and the material was applied to the entire back area under occlusion created by application of a woven pad and a rubber dental dam over the application site. The material was left in contact with the skin for 24 hours, then washed off. Animals were assessed for skin irritation upon patch removal and for the next few days. Dermal toxicity and lethality were assessed by recording any clinical signs which subsequently evolved over a period of 10 days. As with the oral studies, weight loss was considered a sign of toxicity and an indication of absorption of the test chemical. The validity of these studies rests on the fact that, even though few animals were tested, the exposure regimen was far in excess of current guidelines. For example, for dermal toxicity, the current limit dose is 2000 mg/kg, applied for 24 hours; using the Kodak method, the 20 gm/kg dose used was 10 times greater than is currently recommended. Therefore, the exposure regimen in the Kodak studies used up to 40 times more material applied six times longer. This resulted in an exposure 240 times more severe than currently required in skin irritation studies conducted using international Guidelines.

Duration of exposure:

24 hours

Doses:

5, 10, 20 ml/kg

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

> 20 mL/kg bw

Based on:

test mat.

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

> 20 mL/kg bw

Based on:

test mat.

Mortality:

none observed

Clinical signs:

other: slight to moderate erythema was observed on days 1-3.

Applicant's summary and conclusion

Interpretation of results:

relatively harmless

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

Acute dermal LD50 for 1,3,5-triisopropylbenzene (TIPB) for both male and female guinea pigs was determined to be greater than 20 mL/kg.

Executive summary:

Acute dermal toxicity of 1,3,5 -triisopropylbenzene (TIPB) was evaluated in guinea pigs using a limit dose of 20 mL/kg. Hair was clipped from the back of 5 male and 5 female guinea pigs, and TIPB was applied to the entire back under an occlusive dressing for 24 hours and then washed off. There was no mortality observed during the course of this study, and therefore the acute oral LD50 for TIPB is considered to be > 20 mL/kg for both male and female guinea pigs.