Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
05 JAN - 24 JAN 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Substance type: C12-C14 Fatty alkoholpolyethylenglycolether with 2 EO
- Physical state: liquid
- Analytical purity: 73% a.s. (w/w)
- Lot/batch No.: Pt. 24, Tank 7

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil (if used): Stock solution (0.23 g substance in 20 g quarz sand) was mixed with 1680 g steril substrate to a concentration of 100 mg/kg dry substrate.

Test organisms

open allclose all
Species:
Triticum aestivum
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
- Common name: wheat
- Plant family: Poacea
Species:
Brassica alba
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: White mustard
- Plant family: Brassicaceae
Species:
Lepidum sativum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: Garden cress
- Plant family: Brassicaceae

Study design

Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
yes
Total exposure duration:
19 d

Test conditions

Test temperature:
20 - 24 °C
pH:
5.0 - 7.5
Moisture:
approximately 60%
Details on test conditions:
TEST SYSTEM
- Test container (type, material, size): plastic pots, diameter 6 cm (bottom) and 8 cm (top), 6 cm (height)
- Amount of soil: approximately 100 g substrate
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 4



SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: LUFA soil Speyer (Sp. 2.3)
- Soil texture (if natural soil): particle < 20 µm: 10 - 20%
- Organic carbon (%): < 1.5%



GROWTH CONDITIONS
- Photoperiod: 16 h day / 8 h night
- Light intensity and quality: day: 1500 - 2000 lux, night: approximately 50 lux
- Day/night temperatures: 20 - 24 °C
- Relative humidity (%): 90% - 100%
- Interval of applications: 1 - 2 times per week

Nominal and measured concentrations:
100 mg/kg soil
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Species:
Triticum aestivum
Duration:
19 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks:
and growth
Species:
Brassica alba
Duration:
19 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks:
and growth
Species:
Lepidum sativum
Duration:
19 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks:
and growth
Details on results:

SEEDLING EMERGENCE
- Percent seedling emergence: Triticum aestivum: 100 mg/kg: 90% (85% control), Brassica alba: 100 mg/kg: 80% (80% control), Lepidum sativum: 100 mg/kg: 95% (control: 95%)
- Fresh weight: Triticum aestivum: 100 mg/kg: 357 mg (389 mg control), Brassica alba: 100 mg/kg: 248 mg (228 mg control), Lepidum sativum: 100 mg/kg: 71 mg (control: 61 mg)

Applicant's summary and conclusion